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首页> 外文期刊>American Journal of Experimental Agriculture >Somatic Embryo Formation from Co-cultivated Protoplasts of Brassica rapa & B. juncea
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Somatic Embryo Formation from Co-cultivated Protoplasts of Brassica rapa & B. juncea

机译：芸苔和芥菜共生原生质体的体细胞胚形成

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Aims: The objectives of this experiment was to establish initial steps of somatic embryogenesis from the fused protoplasts between two Brassica species : Brassica rapa L. (var. Agrani, AA, 2n=20) and Brassica juncea L. (var. BINA Sharisha-7 and var. BINA Sharisha-8, AABB, 2n=36). Study Design: The experiment was carried out in completely randomized design with three replications for each hormone treatment of each variety combination. Place and Duration of Study: An experiment was conducted in growth room of tissue culture laboratory at the Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh during the period from April, 2013 to May, 2014. Methodology: Mesophyll protoplasts were isolated from the young leaves of aseptically grown plants. Variety combinations for protoplasts fusion were: Agrani+BINA Sharisha-7 and Agrani+ BINA Sharisha-8. 100% polyethylene glycol-400 was used as a fusogen. Pellieter different compositions of liquid media were used for protoplasts cultured, microcalli, calli and somatic embryo formation. Results: Fused products were cultured in a modified Pellieter B liquid media with different combinations of auxins and cytokinin for callus induction. A medium having 1 mg L-1 NAA + 0.5 mg L-1 2,4-D produced micro-calli of 518 μm diameter on 15 days after dark treatment. The same hormone combinations produced 1.6 mm visible calli after 24 days of incubation. After 82 days of culture, a medium composition of 0.5 mg L-1 BAP + 0.5 mg L-1 NAA resulted in 76% somatic embryo formation. Agrani+BINA Sarisha-7 produced significantly higher somatic embryos compared to Agrani+BINA Sarisha-8. Conclusion: Our results might be helpful for developing a complete and efficient protocol of somatic hybridization in Brassica species.
机译：目的：本实验的目的是从两个芸苔属（Brassica rapa L.（变种Agrani，AA，2n = 20）和芸苔芥菜L.（变种BINA Sharisha-）之间的融合原生质体建立体细胞胚发生的初始步骤。 7和var BINA Sharisha-8，AABB，2n = 36）。研究设计：实验以完全随机的设计进行，每种激素组合的每种激素处理均重复3次。研究的地点和持续时间：2013年4月至2014年5月，在孟加拉国农业大学遗传与植物育种系Mymensingh的组织培养实验室的生长室进行了一项实验。方法：叶肉原生质体分离自无菌种植植物的幼叶。用于原生质体融合的品种组合为：Agrani + BINA Sharisha-7和Agrani + BINA Sharisha-8。使用100％聚乙二醇400作为融合剂。使用不同浓度的液体培养基来培养原生质体，微愈伤组织，愈伤组织和体细胞胚。结果：融合产物在改良的Pellieter B液体培养基中培养，生长素和细胞分裂素的不同组合可诱导愈伤组织。在黑暗处理后15天，具有1mg L -1 NAA + 0.5mg L -1 2，4-D的培养基产生了直径为518μm的微愈伤组织。孵育24天后，相同的激素组合产生1.6毫米可见的愈伤组织。培养82天后，0.5 mg L -1 BAP + 0.5 mg L -1 NAA的培养基组成导致76％的体细胞胚形成。与Agrani + BINA Sarisha-8相比，Agrani + BINA Sarisha-7产生了更高的体细胞胚。结论：我们的结果可能有助于开发完整和有效的芸苔属植物体细胞杂交方案。

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