• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Agriculture and Biology Journal of North America >Contribution in isolation and identification of some pathogenic fungi from wheat seeds, and evaluation of antagonistic capability of Trichoderma harzianum against those isolated fungi in vitro.
【24h】

Contribution in isolation and identification of some pathogenic fungi from wheat seeds, and evaluation of antagonistic capability of Trichoderma harzianum against those isolated fungi in vitro.

机译：从小麦种子中分离和鉴定某些病原真菌的贡献，以及哈茨木霉对体外分离的真菌的拮抗能力的评估。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of this study was to isolate and to identify the pathogenic fungi accompanying interior in hard wheat seeds, and to evaluate the antagonism capability in vitro of (Trichoderma harzianum) against those pathogenic isolated fungi. Three samples of local hard wheat seeds (Triticum durum Desf) were brought from Oum-elbouaghi (Algeria) silos yields, follower for varieties: Vitron, Waha, and Cirta. ).They were stored for one year. The results of isolation on potato-dextrose-agar (PDA) medium and after identification of fungal accompanying interior in the seeds clarified about (83) fungus isolates following for species next : Fusarium acuminatum (1 isolate) in a ratio of (1.2%), and Alternaria(82 isolates) with a ratio of (98.795 %).The Alternaria isolates apportioned among two species: Alternaria alternata with 39 isolates(12 isolates for: Alternaria alternata1 and 27 isolates for : Alternaria alternata2) and Alternaria infectoria with 43 isolates).And, besides it was clearly seen that the Cirta variety was a lowest infected with fungi(19 isolates) in ratio of (22.89 %), and both varieties Waha and Vitron with(32 isolates)for each one in a ration equal (38.554 %) . The results of the antagonistic capability in vitro of (Trichoderma harzianum) against isolated fungi, showed that: 1- The direct confrontation in vitro of Trichoderma harzianum against the different fungus isolates on PDA medium, showed that: a- In the fourth day of the experiment, Trichoderma harzianum inhibited the mycelia growth of different pathogenic fungi in dual cultures, with different ratios, it was equal to: 6.66% forFusarium acuminatum, and 23%, 26.66% and 40% for Alternaria alternata1, Alternaria alternata2, and Alternaria infectoria, respectively. b-The microscopic observations of the different interactions hyphal between Trichoderma harzianum and different fungus isolates, showed that the antagonistic fungus affected on Alternaria species with the decomposition phenomenon (Lyses), where it analyzed the mycelia and spores of Alternaria altrenata2 and Alternaria infectoria, while it analyzed the mycelia of Alternaria alternata1, compared with control. 2- The remote confrontation in vitro of Trichoderma harzianum against the different fungus isolates on PDA medium showed that : a- The volatile metabolic substances of Trichoderma harzianum affected the growth of the different pathogenic fungi, with different ratios over the seven days of treatment, compared with control.b- The microscopic observations Noted that the volatile metabolic substances of Trichoderma harzianum affected some isolated fungi with mycelia analysis prevent the spore's formation of the Alternaria alternata2, while stopped the spore formation of Fusarium acuminatum, compared with control.
机译：本研究的目的是分离和鉴定硬质小麦种子内部伴生的致病真菌，并评估哈茨木霉（Trichoderma harzianum）对这些致病真菌的体外拮抗能力。从Oum-elbouaghi（阿尔及利亚）的筒仓产量中提取了三个本地硬质小麦种子（Triticum durum Desf）样品，该品种的追随者是Vitron，Waha和Cirta。 ）。它们被保存了一年。在马铃薯葡萄糖琼脂（PDA）培养基上分离结果以及鉴定种子中的真菌伴生内部后，澄清了约（83）种真菌分离物，继之为种：镰刀菌（1分离物），比例为（1.2％） ，和链格孢属（82菌株），比例为（98.795％）。链格孢属菌株在两个物种之间分配：链格孢属39种菌株（12种菌株：链格孢菌1和27种菌株：链格孢菌2）和链格孢菌43种。除此之外，还清楚地看到Cirta变种感染真菌的比例最低（19个分离株），比例为（22.89％），Waha和Vitron变种的比例分别为（32个分离株）（ 38.554％）。哈茨木霉（Trichoderma harzianum）对离体真菌的体外拮抗作用的结果表明：1- 1-哈茨木霉（Trichoderma harzianum）在PDA培养基上对不同真菌分离物的直接对抗表明：a-在第4天实验中，哈茨木霉菌在双重培养中抑制不同病原真菌的菌丝体生长，其比率不同，等于：尖孢镰刀菌为6.66％，链格孢菌，链格孢菌和链格孢菌分别为23％，26.66％和40％，分别。 b-对哈茨木霉和不同真菌分离物之间不同菌丝的相互作用的显微镜​​观察表明，拮抗性真菌以分解现象（Lyses）影响了链格孢属，分析了链格孢和链格孢和链格孢的菌丝和孢子，而与对照相比，它分析了链格孢菌的菌丝体。 2-哈茨木霉菌与PDA培养基上不同真菌分离物的体外远程对抗表明：a-哈茨木霉菌的挥发性代谢物质在治疗7天中以不同的比率影响了不同病原真菌的生长。 b-显微镜观察结果表明，与对照相比，哈氏木霉的挥发性代谢物质通过菌丝体分析影响了一些分离的真菌，阻止了孢霉的形成，同时阻止了枯萎镰刀菌的孢子形成。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号