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首页> 外文期刊>Annals of Maxillofacial Surgery >Comparison of attachment and proliferation of human gingival fibroblasts on different collagen membranes
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Comparison of attachment and proliferation of human gingival fibroblasts on different collagen membranes

机译：人牙龈成纤维细胞在不同胶原膜上附着和增殖的比较

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Background and Aim: Human gingival fibroblasts cultured on collagen membrane as an alternative treatment method used in tissue regeneration can lead to improved results in root coverage. The aim of this study was to evaluate the human gingival fibroblast proliferation and adhesion cultured on three types of collagen membranes. Materials and Methods: In this in vitro study, first-line human gingival fibroblast cells (HGF1-RT1) prepared and cultured on three membranes, including porcine pericardium (PP) (Jason, Botiss dental), human pericardium (HP) (Regen, Faravardeh Baft Iranian), and glutaraldehyde cross-linked (GC) (BioMend Extend, Zimmer Dental). Cell survival was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) after 24, 48, and 72 h and 7 days. Furthermore, morphology and adhesion of cells on the membrane were evaluated after 1 and 7 days by electron microscopy (scanning electron microscopy [SEM]). Statistical analysis was performed using two-way ANOVA with a significance level of 0.05. Results: Based on the results of MTT, cell survival on HP and PP membranes after 7 days significantly increased (P P = 0.031). Cell survival on HP and PP membranes did not differ (P = 1) and was more than GC (P Conclusion: The results of this study showed that natural collagen membranes (HP and PP) similarly support proliferation and adhesion of gingival fibroblasts. Survival and adhesion of gingival fibroblasts on cross-linked collagen membrane was less than two other membranes.
机译：背景与目的：在胶原膜上培养人牙龈成纤维细胞，作为组织再生中的一种替代治疗方法，可以改善牙根覆盖率。这项研究的目的是评估在三种类型的胶原膜上培养的人牙龈成纤维细胞的增殖和粘附。材料和方法：在这项体外研究中，制备并在三层膜上培养并培养第一线人牙龈成纤维细胞（HGF1-RT1），包括猪心包膜（PP）（Jason，Botiss dental），人心包膜（HP）（Regen， Faravardeh Baft伊朗）和戊二醛交联（GC）（BioMend Extend，齐默尔牙科）。在24、48、72小时和7天后，使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四唑（MTT）评估细胞存活率。此外，在1和7天后通过电子显微镜（扫描电子显微镜[SEM]）评估细胞在膜上的形态和粘附。统计分析使用两因素均方差分析进行，显着性水平为0.05。结果：根据MTT的结果，HP和PP膜在7天后的细胞存活率显着增加（P P = 0.031）。在HP和PP膜上的细胞存活率没有差异（P = 1），并且比GC更高（P结论：这项研究的结果表明，天然胶原膜（HP和PP）同样支持牙龈成纤维细胞的增殖和粘附。牙龈成纤维细胞在交联的胶原膜上的粘附力小于其他两个膜。

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