...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Annals of microbiology >Identification and characterization of the endophytic bacterium Bacillus atrophaeus XW2, antagonistic towards Colletotrichum gloeosporioides
【24h】

Identification and characterization of the endophytic bacterium Bacillus atrophaeus XW2, antagonistic towards Colletotrichum gloeosporioides

机译：鉴定和表征内生细菌Atrophaeus XW2，对炭疽菌具有拮抗作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacillus atrophaeus XW2 was isolated from healthy poplar leaves and exhibited a strong inhibitory effect against Colletotrichum gloeosporioides, a predominant fungus causing poplar anthracnose. The culture filtrate and bacterial lysate of XW2 were antagonistic against hyphal growth and spore germination. The average diameter of the inhibition zones of hyphal growth were 22.3?mm for the culture filtrate and 12.1?mm for the bacterial lysate. The average inhibition rates of spore germination after 24?h were 94.6?% for the culture filtrate and 88.8?% for the bacterial lysate. In the presence of both culture extracts, hyphae developed vacuoles and swelling, and abnormalities were observed in 100?% of germinated spores. Lipopeptides isolated from culture filtrate and bacterial lysate had high antifungal activities against C. gloeosporioides. The average diameter of the inhibition zones were 23.2?mm for culture filtrate and 11.8?mm for bacterial lysate. Crude proteins isolated from culture filtrate and bacterial lysate also showed good inhibition qualities. The average diameters for the zones of inhibition were 22.3?mm for culture filtrate and 13.4?mm for bacterial lysate. Volatiles produced by XW2 inhibited hyphal growth of C. gloeosporioides by 60.2?% and were antagonistic against the germination of C. gloeosporioides spores after 3?days. Greenhouse studies revealed that XW2 had a 49.1?% efficacy in controlling poplar anthracnose 12?days after exposure to the pathogen. We consider that Bacillus atrophaeus XW2 is a promising natural biocontrol agent for use against C. gloeosporioides.
机译：从健康的杨树叶片中分离出萎缩芽孢杆菌XW2，并表现出对Colletotrichum gloeosporioides的强抑制作用，Colletotrichum gloeosporioides是引起杨炭疽病的主要真菌。 XW2的培养滤液和细菌裂解液对菌丝生长和孢子萌发具有拮抗作用。菌丝生长抑制区的平均直径，培养滤液为22.3mm，细菌裂解液为12.1mm。培养液滤液在24小时后的平均孢子萌发抑制率为94.6％，细菌裂解液为88.8％。在两种提取物均存在的情况下，菌丝形成了液泡和肿胀，并且在100％的发芽孢子中观察到异常。从培养物滤液和细菌裂解物中分离出的脂肽对球孢梭菌具有较高的抗真菌活性。培养滤液的抑菌圈平均直径为23.2？mm，细菌裂解液的抑菌圈平均直径为11.8？mm。从培养滤液和细菌裂解物中分离出的粗蛋白也显示出良好的抑制作用。培养滤液的抑菌圈平均直径为22.3毫米，细菌裂解液的平均直径为13.4毫米。 XW2产生的挥发物抑制了C. gloeosporioides的菌丝生长60.2％，并在3 d后对抗C. gloeosporioides孢子的萌发。温室研究表明，XW2在暴露于病原体12天后，对控制杨炭疽病的功效为49.1％。我们认为，萎缩芽孢杆菌XW2是一种有前途的天然生物防治剂，可用于对抗C. gloeosporioides。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号