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首页> 外文期刊>Annals of microbiology >Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product
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Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product

机译：白蘑菇生产系统中的微生物从堆肥和肠衣到市场上出售的包装产品

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The aim of the study was to investigate microbial succession in the mushroom supply chain from compost, casing to fruit body formation and mushroom growth to the point of harvest, packing and point of sale. The microbial population dynamics of compost, casing and mushrooms were determined using a plate count technique, denaturing gradient gel electrophoresis (DGGE) and sequencing of 16S and 18S rDNA. Plating revealed greater abundance of bacteria, fungi and yeasts in mushroom compost compared to casing and fresh mushroom samples. The viable count method also showed that bacteria and yeasts increased significantly after harvest and during cold storage. Sequencing revealed a more diverse culturable bacterial population in casing and on the mushrooms than in the compost. Phylogenetic analysis revealed a general trend of grouping of species from the same sources. In contrast, a higher microbial diversity was recorded in compost when using the DGGE method, which reflects both cultural and non-culturable microorganisms. For compost and casing bacteria studied using DGGE, several species formed separate lineages, demonstrating highly diverse communities in these samples. Fungi were shown to be less abundant and less diverse compared to bacteria and yeasts. The study provides baseline knowledge of microbial populations and succession trends in mushroom production systems using viable and non-viable methods. The information provided in this study may be useful for microbial ecology studies and to identify and develop biocontrol systems for pathogen control during production or to enhance pinning stimulation by knowing when to apply Pseudomonas spp. to ensure increased yield. Finally, an insight is provided into microbial survival during cold storage and marketing of mushrooms. Potential antagonistic populations known to prevent spoilage, quality deterioration and extend shelf life are listed in this paper.
机译：这项研究的目的是研究蘑菇堆肥，堆肥，肠衣到子实体的形成以及蘑菇生长到收获，包装和销售点的微生物演替。使用平板计数技术，变性梯度凝胶电泳（DGGE）以及16S和18S rDNA测序确定堆肥，肠衣和蘑菇的微生物种群动态。电镀显示，与肠衣和新鲜蘑菇样品相比，蘑菇堆肥中细菌，真菌和酵母的含量更高。可行的计数方法还显示，收获后和冷藏期间细菌和酵母菌显着增加。测序表明，与堆肥相比，肠衣和蘑菇上可培养的细菌种群更多。系统发育分析揭示了来自相同来源的物种分组的总体趋势。相反，当使用DGGE方法时，堆肥中记录到较高的微生物多样性，这既反映了培养的微生物又反映了不可培养的微生物。对于使用DGGE研究的堆肥和肠衣细菌，几种物种形成了不同的谱系，表明这些样品中的群落高度不同。与细菌和酵母菌相比，真菌的丰富度和多样性较低。该研究使用可行和不可行的方法提供了蘑菇生产系统中微生物种群和演替趋势的基础知识。这项研究中提供的信息可能对微生物生态学研究以及在生产过程中识别和开发用于病原体控制的生物控制系统或通过知道何时应用假单胞菌属来增强钉扎刺激有用。以确保提高产量。最后，我们对蘑菇的冷藏和销售过程中的微生物存活情况提供了见解。本文列出了已知可以防止变质，质量下降和延长保质期的潜在拮抗种群。

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