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首页> 外文期刊>Analytical methods >A T7 exonuclease-RCA dual amplification system for high-sensitivity and high-selectivity analysis of microRNA
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A T7 exonuclease-RCA dual amplification system for high-sensitivity and high-selectivity analysis of microRNA

机译：T7核酸外切酶-RCA双扩增系统，用于microRNA的高灵敏度和高选择性分析

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摘要

We developed a novel microRNA (miRNA) detection method with high-sensitivity and high-selectivity, based on rolling circle amplification (RCA) and the T7 exonuclease-assisted cyclic enzymatic amplification method. T4 DNA ligase is responsible for the selectivity, RCA enables the target miRNA to trigger DNA replication, and T7 Exo hydrolyzes the signal probe to further enhance the signal. This approach can detect miRNA with concentrations as low as 7 fM and can distinguish different miRNAs in the same family. In practical applications, this scheme can distinguish cancer cells by determining their let-7b miRNA content. This sensitive and selective detection scheme could therefore be used for early clinical diagnosis of cancers like those of the liver and lung.
机译：我们基于滚环扩增（RCA）和T7核酸外切酶辅助的循环酶扩增方法，开发了一种具有高灵敏度和高选择性的新型microRNA（miRNA）检测方法。 T4 DNA连接酶负责选择性，RCA使目标miRNA触发DNA复制，T7 Exo水解信号探针以进一步增强信号。这种方法可以检测低至7 fM的miRNA，并且可以区分同一家族中的不同miRNA。在实际应用中，该方案可以通过确定let-7b miRNA含量来区分癌细胞。因此，这种灵敏且选择性的检测方案可用于癌症如肝癌和肺癌的早期临床诊断。

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