Can we use LDL instead of egg yolk in BotuCrio? extender to cryopreserve sperm from the Mangalarga Marchador stallion?

Paola Pereira das Neves Snoeck; Tainá Hortência Oliveira Pessoa; Marcelo Gon?alves Sousa Pereira; Inara Cristina Leal Bastos; Maria Isabel Vaz de Melo

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Can we use LDL instead of egg yolk in BotuCrio? extender to cryopreserve sperm from the Mangalarga Marchador stallion?

机译：我们可以在BotuCrio中使用LDL代替蛋黄吗？ Mangalarga Marchador种马的冷冻精子的延伸剂？

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The objective of this study was to compare the BotuCrio? extender with the Merk - egg yolk and the INRA 82 modified by the inclusion of acetamide, methyl cellulose and trehalose in substitution of glycerol for freezing equine semen. The semen was diluted after centrifugation to obtain 100 x 106 of sperm/ml in: BotuCrio? (control); Merk - egg yolk or INRA 82 modified (Experiment 1). The extended semen was packaged in 0.5 ml straws, cooled and frozen in a freezing machine. The control extender was superior in preserving the motility, VCL, VSL, VAP, LIN, STR and the BCF when compared to the Merk - egg yolk and INRA 82 modified (P 0.05). The fertility rate after AI with frozen semen in BotuCrio LDL was 37.5%.

机译：这项研究的目的是比较BotuCrio？带有Merk-蛋黄和INRA 82的补充剂，通过加入乙酰胺，甲基纤维素和海藻糖代替甘油来代替冷冻马精液。离心后将精液稀释，以得到100×106的精子/ ml，其形式为：BotuCrio？（控制）;默克-蛋黄或改良的INRA 82（实验1）。将延长的精液包装在0.5ml的吸管中，在冷冻机中冷却并冷冻。与Merk-蛋黄和经修饰的INRA 82相比，对照补充剂在保留运动性，VCL，VSL，VAP，LIN，STR和BCF方面表现优异（P 0.05）。 BotuCrio LDL中AI冷冻精液后的受精率为37.5％。

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《Animal Reproduction》 |2019年第2期|共8页
作者
Paola Pereira das Neves Snoeck; Tainá Hortência Oliveira Pessoa; Marcelo Gon?alves Sousa Pereira; Inara Cristina Leal Bastos; Maria Isabel Vaz de Melo;
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中图分类保护生物学;
关键词
spermlipoproteinsamidecryoprotection. equine.;

机译：精脂蛋白酰胺冷冻保护。马。;

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Can we use LDL instead of egg yolk in BotuCrio? extender to cryopreserve sperm from the Mangalarga Marchador stallion?

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