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首页> 外文期刊>Animal Cells and Systems >Butyrate-induced differentiation of PC12 cells to chromaffin cells involves cell adhesion and induction of extracellular proteins and cell adhesion proteins
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Butyrate-induced differentiation of PC12 cells to chromaffin cells involves cell adhesion and induction of extracellular proteins and cell adhesion proteins

机译：丁酸酯诱导的PC12细胞分化为嗜铬细胞涉及细胞粘附以及细胞外蛋白和细胞粘附蛋白的诱导

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摘要

PC12 cells were differentiated into the cells of chromaffin phenotype by butyrate treatment. Cells were aggregated and formed tight cell adhesion. To investigate the molecular change in this differentiation, we examined expression levels of cell adhesion proteins and extracellular proteins during butyrate induced-differentiation of PC12 cells. Integrin β1, integrin α7, E cadherin, VCAM, collagen-I, fibronectin, desmoglein and connexin were increased during differentiation. The levels of clusterin and secreted clusterin were also increased. These increased levels of cell adhesion proteins and extracellular proteins appear to induce cell aggregation and tight cell adhesion. The levels of p21, p27 and p16 were increased probably because of differentiation-related growth arrest during differentiation. Prolonged incubation of butyrate up to 1 day was required for differentiation. Signal transduction pathways for this differentiatiom could not be identified since various inhibitors had no effect. The results showed that butyrate-induced differentiation of PC12 cells to chromaffin cells involves tight cell adhesion and induction of extracellular proteins and cell adhesion proteins.
机译：通过丁酸盐处理将PC12细胞分化为嗜铬素表型细胞。细胞聚集并形成紧密的细胞粘附。为了研究这种分化中的分子变化，我们检查了丁酸酯诱导的PC12细胞分化过程中细胞粘附蛋白和细胞外蛋白的表达水平。在分化过程中，整合素β1，整合素α7，钙粘蛋白E，VCAM，胶原蛋白I，纤连蛋白，桥粒芯蛋白和连接蛋白增加。簇蛋白和分泌簇蛋白的水平也增加。细胞粘附蛋白和细胞外蛋白的这些增加的水平似乎诱导细胞聚集和紧密的细胞粘附。 p21，p27和p16的水平升高可能是由于分化过程中与分化相关的生长停滞。分化需要将丁酸盐长时间孵育至1天。由于各种抑制剂都没有作用，因此无法确定这种差异的信号转导途径。结果表明，丁酸酯诱导的PC12细胞向嗜铬细胞的分化涉及紧密的细胞粘附以及细胞外蛋白和细胞粘附蛋白的诱导。

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