...
首页> 外文期刊>Analytical Sciences >Method Validation by Interlaboratory Studies of Improved Hydrophilic Oxygen Radical Absorbance Capacity Methods for the Determination of Antioxidant Capacities of Antioxidant Solutions and Food Extracts
【24h】

Method Validation by Interlaboratory Studies of Improved Hydrophilic Oxygen Radical Absorbance Capacity Methods for the Determination of Antioxidant Capacities of Antioxidant Solutions and Food Extracts

机译:改进的亲水氧自由基吸收能力方法的实验室间研究方法验证,以确定抗氧化剂溶液和食品提取物的抗氧化剂能力

获取原文
           

摘要

Hydrophilic oxygen radical absorbance capacity (H-ORAC) is a method for evaluating antioxidant capacities of solutions of hydrophilic compounds. In this study, we improved the original method for H-ORAC determination, and evaluated the precision of the two improved methods (methods A and B) by interlaboratory studies using 5 antioxidant solutions and 5 food extracts as test samples. An interlaboratory study of method A, in accordance with the harmonized protocol, demonstrated satisfactory performance (intermediate precision relative standard deviations (RSDint) ranging from 4.6 to 18.8%; the reproducibility relative standard deviations (RSDR) ranging from 7.0 to 21.1%, and the HorRat values ranging from 0.40 to 1.93). However, methodological problems remained, and a further improved method, method B, was thus developed. An interlaboratory study of method B by 5 participating laboratories showed better intermediate precision and reproducibility (RSDint and RSDR ranging from 1.8 to 9.4%, and from 4.4 to 13.8%, respectively), and all HorRat values for the test samples were less than 1.3, suggesting good performance for the H-ORAC measurement.
机译:亲水性氧自由基吸收能力(H-ORAC)是一种评估亲水性化合物溶液的抗氧化能力的方法。在这项研究中,我们改进了H-ORAC测定的原始方法,并通过使用5种抗氧化剂溶液和5种食品提取物作为测试样品的实验室间研究,评估了这两种改进方法(方法A和B)的精度。根据协调协议对方法A进行的实验室间研究表明,性能令人满意(中等精度相对标准偏差(RSDint)在4.6至18.8%之间;可再现性相对标准偏差(RSDR)在7.0至21.1%之间,并且HorRat值范围从0.40到1.93)。但是,仍然存在方法上的问题,因此开发了进一步改进的方法B。由5个参与实验室进行的方法B的实验室间研究显示,中间精度和重现性更好(RSDint和RSDR分别为1.8至9.4%和4.4至13.8%,并且测试样品的所有HorRat值均小于1.3,表明H-ORAC测量的性能良好。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号