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首页> 外文期刊>Analytical Sciences >Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source
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Multimodal Imaging of Living Cells with Multiplex Coherent Anti-stokes Raman Scattering (CARS), Third-order Sum Frequency Generation (TSFG) and Two-photon Excitation Fluorescence (TPEF) Using a Nanosecond White-light Laser Source

机译：使用纳秒白光激光源的多重相干抗焦炭拉曼散射（CARS），三阶总和频率生成（TSFG）和双光子激发荧光（TPEF）的活细胞多峰成像

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The subnanosecond “white-light laser” source has been applied to multimodal, multiphoton, and multiplex spectroscopic imaging (M3 spectroscopic imaging) with coherent anti-Stokes Raman scattering (CARS), third-order sum frequency generation (TSFG), and two-photon excitation fluorescence (TPEF). As the proof-of-principle experiment, we performed simultaneous imaging of polystyrene beads with TSFG and TPEF. This technique is then applied to live cell imaging. Mouse L929 fibroblastic cells are clearly visualized by CARS, TSFG, and TPEF processes. M3 spectroscopic imaging provides various and unique cellular information with different image contrast based on each multiphoton process.
机译：亚纳秒的“白光激光”源已应用于具有相干反斯托克斯拉曼散射（CARS），三阶和频产生（TSFG）以及二阶光子激发荧光（TPEF）。作为原理验证实验，我们用TSFG和TPEF对聚苯乙烯珠进行了同时成像。然后将该技术应用于活细胞成像。小鼠L929的成纤维细胞可以通过CARS，TSFG和TPEF过程清晰地看到。 M3光谱成像基于每个多光子过程提供具有不同图像对比度的各种独特细胞信息。

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