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A Novel Nitrite Biosensor Based on Gold Dendrites with Egg White as Template

机译：基于蛋清为模板的金枝晶的新型亚硝酸盐生物传感器

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Gold dendrites (AuD) were synthesized with egg white as the soft template and a novel nitrite (NO_(2)~(?)) biosensor was fabricated by assembly of a myoglobin (Mb)-L-cysteamine (Cys)-AuD biological hybrid. The results of Fourier transform infrared spectra and UV-visible spectra indicated that Mb retained its original structure in the resulting Mb-Cys-AuD. Electrochemical investigation of the biosensor showed a pair of well-defined, quasi-reversible redox peaks with E _(pa) = ?0.314 V and E _(pc) = ?0.344 V (vs . SCE) in 0.1 M, pH 7.0 sodium phosphate buffered saline at the scan rate of 200 mV/s. The transfer rate constant (k _(s)) was 1.49 s~(?1). The Mb-Cys-AuD showed a good electrochemical catalytic response for the reduction of NO_(2)~(?), with the linear range from 0.5 to 400 μM and the detection limit of 0.3 μM (S/N = 3). The apparent Michaelis–Menten constant (K _(M)~(app)) was estimated to be 0.2 mM. Therefore, the assembled bio-hybrid as a novel matrix opened up a further possibility for study on the design of enzymatic biosensors with potential applications.
机译：以蛋清为软模板合成金树突状细胞（AuD），并通过组装肌红蛋白（Mb）-L-半胱胺（Cys）-AuD生物杂化体制备了新型亚硝酸盐（NO_（2）〜（？））生物传感器。 。傅立叶变换红外光谱和紫外可见光谱的结果表明，Mb在生成的Mb-Cys-AuD中保留了其原始结构。生物传感器的电化学研究显示了一对明确定义的，可逆的氧化还原峰，其中E_（pa）= 0.314 V和E_（pc）= 0.344 V（vs。 SCE）在200 mV / s的扫描速率下在0.1 M，pH 7.0的磷酸钠缓冲盐水中溶解。传递速率常数（k _（s））为1.49 s·（Δ1）。 Mb-Cys-AuD对NO_（2）〜（？）的还原表现出良好的电化学催化响应，线性范围为0.5至400μM，检出限为0.3μM（S / N = 3 ）。表观Michaelis-Menten常数（K_（M）〜（app））估计为0.2 mM。因此，组装后的生物杂交体作为新型基质为研究具有潜在应用的酶促生物传感器的设计开辟了进一步的可能性。

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