Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene

Pedro Carnieli Jr; Juliana Galera Castilho; Rafael de Novaes Oliveira; Paulo Eduardo Brand?o; Helena Beatriz de Carvalho Ruthner Batista

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Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene

机译：通过线粒体DNA细胞色素B基因的测序鉴定参与作为人畜共患病的人畜共患病的不同种类的哺乳动物。

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Introduction: The identification of species that act as reservoirs or hosts of zoonotic agents is essential for control and epidemiological surveillance of the important illness in public health. Identification of the reservoirs for zoonoses can help to clarify how the pathogens are maintained in nature, leading to more effective disease control and avoiding indiscriminate extermination of wild animals. Aims: The objective of this study was to describe the genetic identification of 106 samples isolated from different mammalians species. Methodology: This study was conducted using 106 tissue samples from wild and domestic mammals sent to rabies diagnosis in Pasteur Institute, Brazil. Sequencing of the mitochondrial DNA b gene and Basic Local Alignment Search Tool (BLAST) was used to confirm species identity. Results and Conclusion: By sequencing the mtDNA cyt-b gene 10 orders, 20 families, 34 genera and 38 species of mammalians were identified. In conclusion, the method used at this work was efficient for identification of different species of mammalians. Animals identified at this work with same method, belong to high distance order, as marsupials, chiropters and primates.

机译：简介：识别充当人畜共患病病原体的宿主或宿主的物种对于控制和流行病学监测公共卫生中的重要疾病至关重要。识别人畜共患病的水库可以帮助阐明病原体如何在自然环境中维持，从而更有效地控制疾病并避免野生动物的滥杀滥伤。目的：本研究的目的是描述从不同哺乳动物物种中分离出的106个样品的遗传鉴定。方法：这项研究是使用来自巴西巴斯德研究所狂犬病诊断的野生和家养哺乳动物的106个组织样本进行的。线粒体DNA b基因的测序和基本本地比对搜索工具（BLAST）用于确认物种身份。结果与结论：通过对mtDNA cyt-b基因进行10个测序，鉴定出20个科，34属和38种哺乳动物。总之，这项工作中使用的方法对于识别不同种类的哺乳动物是有效的。用同样的方法在这项工作中鉴定出的动物属于远距离有序动物，如有袋动物，手翅类动物和灵长类动物。

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《Annual Research & Review in Biology》 |2016年第1期|共8页
作者
Pedro Carnieli Jr; Juliana Galera Castilho; Rafael de Novaes Oliveira; Paulo Eduardo Brand?o; Helena Beatriz de Carvalho Ruthner Batista;
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Identification of Different Species of Mammalians Involved in Zoonoses as Reservoirs or Hosts by Sequencing of the Mitochondrial DNA Cytochrome B Gene

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