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首页> 外文期刊>Analytical Sciences >Potential of Capillary Electrophoresis (CE) and Chip-CE with Dual Detection (Capacitively-Coupled Contactless Conductivity Detection (C4D) and Fluorescence Detection) for Monitoring of Nicotine and Cotinine Derivatization
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Potential of Capillary Electrophoresis (CE) and Chip-CE with Dual Detection (Capacitively-Coupled Contactless Conductivity Detection (C4D) and Fluorescence Detection) for Monitoring of Nicotine and Cotinine Derivatization

机译：毛细管电泳（CE）和芯片CE的双重检测（电容耦合非接触电导率检测（C4D）和荧光检测）的潜力，可监测尼古丁和可替宁的衍生

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Capillary electrophoresis (CE) coupled with capacitively-coupled contactless conductivity (C4D) and fluorescence (FD) detectors and chip-CE for monitoring of nicotine and cotinine derivatization was demonstrated. Separation of the substrates and intermediates could be achieved by CE-C4D in 7 min (Rs 2.7) using 45 mM acetic acid (pH 3.0) and this system was applied to detect the intermediate formation. Final fluorescent products could be analyzed by micellar electrokinetic chromatography (MEKC-FD) in 5 mM borate buffer (pH 9.0) containing 10 mM sodium dodecylsulfate (SDS) (%RSD 3.00%). Transferring of MEKC-FD to chip-CE allowed for shorter analysis time (2.5 min) and decreased sample consumption. The chip-CE-FD shows good detection and quantitation limits ( 7.5 μM) and precision (%RSD 2.81%) and was employed to determine nicotine and cotinine in artificial urine. This work reveals the potential of CE and chip-CE with dual detectors as simultaneous, convenient and rapid methods for monitoring pyridine derivatization.
机译：毛细管电泳（CE）与电容耦合的非接触电导率（C4D）和荧光（FD）检测器以及用于监测尼古丁和可替宁衍生化的芯片CE进行了展示。底物和中间体的分离可以通过使用45 mM乙酸（pH 3.0）在7分钟内（Rs> 2.7）通过CE-C4D进行，该系统用于检测中间体的形成。最终的荧光产物可以通过胶束电动色谱法（MEKC-FD）在含有10 mM十二烷基硫酸钠（SDS）（％RSD <3.00％）的5 mM硼酸盐缓冲液（pH 9.0）中进行分析。将MEKC-FD转移到芯片CE可以缩短分析时间（2.5分钟）并减少样品消耗。 CE-FD芯片具有良好的检测和定量限（<7.5μM）和精密度（％RSD <2.81％），可用于测定人造尿液中的尼古丁和可替宁。这项工作揭示了双检测器作为同时，便捷和快速的监测吡啶衍生化方法的CE和芯片CE的潜力。

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