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首页> 外文期刊>Annual Research & Review in Biology >Antimicrobial Activity of Phomopsis sp. ED2 Residing in Medicinal Plant Orthosiphon stamineus Benth
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Antimicrobial Activity of Phomopsis sp. ED2 Residing in Medicinal Plant Orthosiphon stamineus Benth

机译：拟南芥的抗菌活性。 ED2驻留在药用植物Orthosiphon stamineus Benth中

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Aims: To investigate the antimicrobial activity of Phomopsis sp . ED2, endophytic fungus isolated from Orthosiphon stamineus Benth against various pathogenic microorganisms. Study Design: Place and Duration of Study: Industrial Biotechnology Research Laboratory, Universiti Sains Malaysia, Penang, Malaysia, between Dec 2011 to August 2013. Methodology: The endophytic fungus Phomopsis sp . ED2 was cultivated in the culture medium supplemented with the host plant extract. Then the antimicrobial activities of the crude extracts were screened via disc diffusion assay. The minimal inhibitory concentration and minimal lethality concentration of the extract against selected test microorganisms by using broth microdilution assay. The bioactive constituents of the extract were analyzed by using gas chromatography-mass spectroscopy analysis. Results: The ethyl acetate extract of this fungus showed broad spectrum inhibitory activity on both Gram positive and Gram negative bacteria with the minimal inhibitory concentrations ranged from 31.25 to 250 μg/mL. The gas chromatography-mass spectroscopy analysis of the extract showed that the bioactive constituents present in the extract were benzoic acid, lomustine and penicillic acid. Conclusion: The endophytic fungus residing in Orthosiphon stamineus Benth, Phomopsis sp. ED2 exhibited significant antibacterial activity of the test microorganisms. It is a potential source of antibacterial compounds.
机译：目的：研究拟南芥（Phomopsis sp。）的抗菌活性。 ED2，一种从Orthosiphon stamineus Benth分离出来的内生真菌，可抵抗各种病原微生物。研究设计：研究地点和持续时间：马来西亚槟城大学工业生物技术研究实验室，马来西亚，槟城，2011年12月至2013年8月。方法：内生真菌Phomopsis sp。 ED2在补充有宿主植物提取物的培养基中培养。然后通过圆盘扩散试验筛选粗提物的抗菌活性。通过使用肉汤微稀释测定法，提取物对选定的测试微生物的最小抑制浓度和最小致死浓度。通过使用气相色谱-质谱分析法分析提取物的生物活性成分。结果：该真菌的乙酸乙酯提取物对革兰氏阳性和革兰氏阴性细菌均表现出广谱抑制活性，最低抑制浓度范围为31.25至250μg/ mL。提取物的气相色谱-质谱分析表明，提取物中存在的生物活性成分是苯甲酸，洛莫司汀和青霉酸。结论：内生真菌存在于拟南芥Stethus Benth，Phomopsis sp。中。 ED2对测试微生物表现出显着的抗菌活性。它是抗菌化合物的潜在来源。

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