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Evaluation of the effect of indigenous mycogenic silver nanoparticles on soil exo-enzymes in barite mine contaminated soils

机译:评价原生致真菌银纳米颗粒对重晶石矿山污染土壤中土壤外酶的影响

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摘要

The biosynthesis of nanoparticles has received increasing attention due to the growing need to develop safe, cost-effective and environmentally friendly technologies for nanoscale materials synthesis. In this report, silver nanoparticles (AgNPs) were synthesized by treating aqueous Ag+ ions with the culture supernatants of indigenous fungal species of Fusarium solani isolated from barite mine contaminated soils. The formation of AgNPs might be an enzyme-mediated extracellular reaction process. The localized surface plasmon resonance of the formed AgNPs was recorded using UV–VIS spectrophotometer and was characterized using the techniques transmission electron microscopy, particle size analyzer, Fourier transform-infrared spectroscopy (FT-IR), particle size (dynamic light scattering) and zeta potential. The synthesized AgNPs were stable, polydispersed with the average size of 80?nm. FT-IR spectra reveals that proteins and carboxylic groups present in the fungal secrets might be responsible for the reduction and stabilization of the silver ions. Applied to the barite mine contaminated soils, concentration of AgNPs and incubation period significantly influences the soil exo-enzymatic activities, viz., urease, phosphatase, dehydrogenase and β-glucosidase. To the best of our knowledge, this is the first report on this kind of work in barite mine contaminated soils
机译:由于开发纳米​​级材料合成的安全,具有成本效益和环境友好技术的需求不断增长,纳米颗粒的生物合成受到越来越多的关注。在此报告中,通过用重晶石矿山污染土壤中分离的茄形镰刀菌(Fusarium solani)本地真菌物种的培养上清液处理Ag + 水溶液,合成了银纳米颗粒(AgNPs)。 AgNPs的形成可能是酶介导的细胞外反应过程。使用UV-VIS分光光度计记录形成的AgNPs的局部表面等离子体共振,并使用透射电子显微镜,粒度分析仪,傅立叶变换红外光谱(FT-IR),粒度(动态光散射)和ζ表征技术潜在。合成的AgNP稳定,多分散,平均大小为80nm。 FT-IR光谱表明,真菌分泌物中存在的蛋白质和羧基可能是导致银离子还原和稳定的原因。 AgNPs的浓度和潜伏期应用于重晶石矿山污染的土壤时,会显着影响土壤的外酶活性,即脲酶,磷酸酶,脱氢酶和β-葡萄糖苷酶。据我们所知,这是在重晶石矿山污染土壤中进行此类工作的第一份报告

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