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首页> 外文期刊>Applied Nanoscience >Extracellular biosynthesis of silver nanoparticle using Streptomyces sp. 09 PBT 005 and its antibacterial and cytotoxic properties
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Extracellular biosynthesis of silver nanoparticle using Streptomyces sp. 09 PBT 005 and its antibacterial and cytotoxic properties

机译：使用链霉菌sp。的胞外生物合成银纳米颗粒。 09 PBT 005及其抗菌和细胞毒性

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The application of microorganisms for the synthesis of nanoparticles as an eco-friendly and promising approach is welcome due to its non-toxicity and simplicity. The aim of this study was to synthesize silver nanoparticle using Streptomyces sp. (09 PBT 005). 09 PBT 005 was isolated from the soil sample of the agriculture field in Vengodu, Thiruvannamalai district, Tamil Nadu, India. 09 PBT 005 was subjected to molecular characterization by 16S rRNA sequence analysis. It was found that 09 PBT 005 belonged to Streptomyces sp. The isolate Streptomyces sp. 09 PBT 005 was inoculated in fermentation medium and incubated at 30?oC for 12?days in different pH conditions. The 0.02 molar concentration showed good antibacterial activity against Gram-positive and Gram-negative bacteria at pH-7. The synthesis of silver nanoparticles was investigated by UV–Vis spectroscopy, scanning electron microscopy and Fourier Transform Infrared analysis. The synthesized AgNPs sizes were found to be in the dimensions ranging between 198 and 595?nm. The cytotoxicity of the synthesized nanoparticles was studied against A549 adenocarcinoma lung cancer cell line. It showed 83.23?% activity at 100?μl with IC 50 value of 50?μl. This method will be useful in the biosynthesis of nanoparticles
机译：由于其无毒且简单，将微生物用于纳米颗粒的合成作为一种生态友好和有希望的方法受到欢迎。这项研究的目的是使用链霉菌（Streptomyces sp。）合成银纳米颗粒。 （09 PBT 005）。 09 PBT 005是从印度泰米尔纳德邦Thiruvannamalai区Vengodu的农业土壤样品中分离出来的。通过16S rRNA序列分析对09 PBT 005进行了分子表征。发现09 PBT 005属于链霉菌属。分离链霉菌。将09 PBT 005接种到发酵培养基中，并在不同pH条件下于30°C孵育12天。 0.02摩尔浓度在pH-7时对革兰氏阳性和革兰氏阴性细菌表现出良好的抗菌活性。通过紫外-可见光谱，扫描电子显微镜和傅立叶变换红外分析研究了银纳米颗粒的合成。发现合成的AgNP的尺寸在198和595nm之间。研究了合成的纳米颗粒对A549腺癌肺癌细胞的细胞毒性。在100？μl时显示83.23％的活性，IC 50值为50？μl。该方法将对纳米颗粒的生物合成有用。

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