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首页> 外文期刊>Applications in plant sciences. >Characterization of Microsatellite Loci for an Australian Epiphytic Orchid, Dendrobium calamiforme, Using Illumina Sequencing
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Characterization of Microsatellite Loci for an Australian Epiphytic Orchid, Dendrobium calamiforme, Using Illumina Sequencing

机译：使用照度测序表征澳大利亚附生兰花石end石cal的微卫星基因座

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Premise of the study: Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon. Methods and Results: Nineteen microsatellite loci were identified from an Illumina paired-end shotgun library of D. calamiforme. Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ～80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380. Conclusions: These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history.
机译：研究的前提：为附生铅笔兰花石cal兰开发了微卫星基因座，用于对该澳大利亚分类群的种群遗传和系统地理学研究。方法和结果：从D. calamiforme的Illumina配对末端shot枪文库中鉴定出19个微卫星基因座。对来自五个种群的24个个体的多态性和遗传多样性进行了评估，这些个体之间的最大距离为〜80 km。所有基因座均为多态性，每个基因座有2至14个等位基因，预期杂合度为0.486至0.902，同一性值的可能性为0.018至0.380。结论：这些新颖的标记物将成为研究遗传多样性水平以及基因流，遗传结构和系统地理史的宝贵工具。

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