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首页> 外文期刊>Applications in plant sciences. >Transcriptome-Facilitated Development of SNPs for the Sonoran Desert Rock Fig, Ficus petiolaris (Moraceae)
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Transcriptome-Facilitated Development of SNPs for the Sonoran Desert Rock Fig, Ficus petiolaris (Moraceae)

机译：转录组促进Sonoran沙漠岩无花果榕（榕科）的SNP的发展。

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Premise of the study: Single-nucleotide polymorphism (SNP) primers were developed for a native North American desert fig, Ficus petiolaris (Moraceae), to provide markers for population genetic studies designed to quantify patterns of gene flow across a complex landscape. Methods and Results: Transcriptome sequencing and bioinformatic protocols were implemented to discover SNPs in single-copy protein-coding genes. Multiplexes of 30 nuclear and 24 organellar (chloroplast and mitochondrial) SNPs were selected for primer development and genotyping on the Sequenom MASSArray System. Of these 54 loci, 49 reliably amplified across a panel of 96 F. petiolaris individuals. Conclusions: This study has provided SNP primers that can be applied in future studies investigating population genetics of F. petiolaris and its coevolution with associated pollinating and nonpollinating fig wasps.
机译：研究的前提：为北美本地无花果无花果榕（Moraceae）开发了单核苷酸多态性（SNP）引物，为群体遗传研究提供了标记，旨在量化复杂景观中基因流的模式。方法和结果：进行了转录组测序和生物信息学方案，以发现单拷贝蛋白质编码基因中的SNP。选择了30个核和24个细胞器（叶绿体和线粒体）SNP的多重序列，以在Sequenom MASSArray系统上进行引物开发和基因分型。在这54个基因座中，有49个在96个细叶镰刀菌个体中可靠地扩增。结论：本研究提供了SNP引物，可用于未来研究小叶镰刀菌的种群遗传及其与相关的授粉和非授粉无花果蜂共同进化的研究。

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