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Generation and Performance of R132H Mutant IDH1 Rabbit Monoclonal Antibody

机译：R132H突变IDH1兔单克隆抗体的产生和性能

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Isocitrate dehydrogenase 1 (IDH1) gene mutations have been observed in a majority of diffuse astrocytomas, oligodendrogliomas, and secondary glioblastomas, and the mutant IDH1 R132H is detectable in most of these lesions. By specifically targeting the R132H mutation through B-cell cloning, a novel rabbit monoclonal antibody, MRQ-67, was produced that can recognize mutant IDH1 R132H and does not react with the wild type protein as demonstrated by Enzyme-linked immunosorbent assay (ELISA) and Western blotting. Through immunohistochemistry, the antibody is able to highlight neoplastic cells in glioma tissue specimens, and can be used as a tool in glioma subtyping. Immunohistochemistry (IHC) detection of IDH1 mutant protein may also be used to visualize single infiltrating tumor cells in surrounding brain tissue with an otherwise normal appearance.
机译：在大多数弥漫性星形细胞瘤，少突胶质细胞瘤和继发性胶质母细胞瘤中均观察到了异柠檬酸脱氢酶1（IDH1）基因突变，并且在大多数这些病变中均可检测到突变体IDH1 R132H。通过B细胞克隆特异性靶向R132H突变，生产了一种新型兔单克隆抗体MRQ-67，可以识别IDH1 R132H突变体，并且不与野生型蛋白反应，如酶联免疫吸附试验（ELISA）所示和蛋白质印迹。通过免疫组织化学，该抗体能够突出神经胶质瘤组织标本中的肿瘤细胞，并可用作神经胶质瘤亚型的工具。 IDH1突变蛋白的免疫组织化学（IHC）检测还可用于以其他正常外观可视化周围脑组织中的单个浸润性肿瘤细胞。

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