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首页> 外文期刊>Aquaculture Reports >Conspecific vitellogenin induces the expression of vg gene in the Indian male walking catfish, Clarias batrachus (Linn.)
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Conspecific vitellogenin induces the expression of vg gene in the Indian male walking catfish, Clarias batrachus (Linn.)

机译：同种卵黄蛋白原诱导印度雄性walking鱼Clarias batrachus（Linn。）的vg基因表达

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To understand the regulatory mechanism of Vg induced vitellogenin synthesis the vg gene expression in Indian male walking catfish, Clarias batrachus was investigated. Semipurified conspecific Vg containing Vg1 and Vg2 in a ratio of 2.7:1.0 was administered into male catfish and vg cDNA (1.1kb) was amplified from total RNA in liver by reverse transcriptase polymerase chain reaction (RT-PCR) using primers designed from published sequence of Clarias macrocephalus. The nucleotide and deduced amino acid sequence of the cDNA shared maximum similarities (98-100%) with the corresponding sequences of known E"2-induced Vg of C. batrachus and C. macrocephalus in the database. A 0.178kb cDNA fragment nested within the 1.1kb DNA was then amplified by real time PCR to evaluate the role of Vg on relative expression of vg gene. The result revealed a significant upregulation (1.79 fold) of vg mRNA at 6h reaching maximum level (9.78 fold) at 24h post Vg injection as compared to the saline control. Similarly, E"2-treatment also showed maximum mRNA expression (7.73 fold) at 24h post injection. The findings suggest that like E"2, Vg itself can induce vg gene expression resulting in a significant increase in plasma Vg levels (9.11+/-0.73mg/ml for Vg1 and 3.02+/-0.28mg/ml for Vg 2) in male catfish where E"2 is lacking. The work provides further opportunity to study the regulatory mechanism of vg gene expression by Vg.
机译：为了了解Vg诱导卵黄蛋白原合成的调控机制，研究了印度male游鱼的vg基因表达。将半纯化的含Vg1和Vg2比例为2.7：1.0的同种Vg施用到雄性cat鱼中，并使用逆转录酶聚合酶链反应（RT-PCR）使用从公开序列设计的引物从肝脏总RNA中扩增vg cDNA（1.1kb）枝大头藻。 cDNA的核苷酸和推导的氨基酸序列与数据库中已知的E” 2诱导的梭状芽胞杆菌和大头梭菌Vg的相应序列具有最大相似性（98-100％）。一个0.178kb cDNA片段嵌套在其中然后通过实时PCR扩增1.1kb DNA，以评估Vg对vg基因相对表达的作用，结果表明，vg mRNA在6h时显着上调（1.79倍），在vg后24h达到最大水平（9.78倍）。与盐水对照相比，E” 2-处理在注射后24小时也显示出最大的mRNA表达（7.73倍）。研究结果表明，与E“ 2一样，Vg本身也可以诱导vg基因表达，从而导致血浆Vg水平显着增加（Vg1为9.11 +/- 0.73mg / ml，Vg 2为3.02 +/- 0.28mg / ml）。缺少E“ 2的雄性fish鱼。这项工作为研究Vg对vg基因表达的调控机制提供了进一步的机会。

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