...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applications in plant sciences. >Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species
【24h】

Development of polymorphic EST‐SSR markers in Itea chinensis (Iteaceae) and cross‐amplification in related species

机译：中华绒螯蟹中多态性EST-SSR标记的建立及相关物种的交叉扩增

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Premise of the StudyWe isolated and characterized 16 expressed sequence tag–simple sequence repeat (EST-SSR) markers in Itea chinensis (Iteaceae), a common evergreen broadleaf tree, for future studies on the genetic diversity and spatial genetic structure of the species. Methods and ResultsBased on transcriptome data of I. chinensis , a total of 36 primer pairs were initially designed and tested. Of these, 16 were successfully amplified and showed clear polymorphism. For these markers, the number of alleles per locus varied from two to 15. The observed and expected heterozygosity ranged from 0 to 0.600 and 0.072 to 0.554, respectively. Furthermore, all loci were successfully cross-amplified in two congeneric species, I. oblonga and I. yangchunensis . ConclusionsThe EST-SSR markers described here can be used to study the genetic diversity and phylogeographic patterns of I. chinensis and other related species in Itea .
机译：摘要研究的前提我们分离并鉴定了常绿阔叶树Itea chinensis（Iteaceae）中的16个表达的序列标签-简单序列重复（EST-SSR）标记，以供将来对该物种的遗传多样性和空间遗传结构研究。方法与结果根据中华I的转录组数据，初步设计和测试了36对引物。其中，成功扩增了16个，并显示出清晰的多态性。对于这些标记，每个基因座的等位基因数量从2到15不等。观察到的和预期的杂合度分别为0到0.600和0.072到0.554。此外，所有基因座均成功地在两个同类中被交叉扩增，即长圆形I.和长春I.。结论本文所述的EST-SSR标记可用于研究伊泰亚和其他相关物种的遗传多样性和系统地理学特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号