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首页> 外文期刊>Applications in plant sciences. >Microsatellite markers for Saussurea polylepis (Asteraceae), a vulnerable continental island species endemic to Korea
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Microsatellite markers for Saussurea polylepis (Asteraceae), a vulnerable continental island species endemic to Korea

机译：朝鲜雪莲​​的一种脆弱的大陆岛屿物种-雪莲（微科）微卫星标记

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Premise Nuclear microsatellite markers were developed for Saussurea polylepis (Asteraceae), a vulnerable species with very limited distribution in a few southwestern continental islands of the Korean peninsula, in order to facilitate future population genetic studies. Methods and Results Based on the Illumina sequence data, a total of 21 microsatellite primer pairs were designed and tested for their suitability. Nineteen of these primers, with two to 11 alleles per locus, were polymorphic in three natural populations of S. polylepis . The levels of expected and observed heterozygosity ranged from 0.000 to 0.842 and 0.000 to 0.933, respectively. Sixteen of these simple sequence repeat (SSR) markers were successfully cross‐amplified in five congeneric species, namely S. gracilis , S. grandifolia , and S. tanakae for all 21 loci, and S. maximowiczii and S. pulchella for 18 loci. Conclusions The SSR markers developed here will be useful for future population genetic studies on S. polylepis and related species.
机译：为朝鲜雪莲（菊科）开发了一种核微卫星标记，这是一种脆弱的物种，在朝鲜半岛的一些西南大岛上分布非常有限，以便于将来进行种群遗传学研究。方法和结果根据Illumina的序列数据，总共设计了21对微卫星引物，并对其适用性进行了测试。这些引物中的十九个，每个基因座具有两个至十一个等位基因，在三个自然链霉菌自然种群中是多态的。预期和观察到的杂合度水平分别为0.000至0.842和0.000至0.933。这些简单的序列重复（SSR）标记中的16个已在5个同类物种中成功交叉扩增，即对于所有21个基因座分别为S. gracilis，S。grandifolia和S. tanakae，以及18个基因座的S. maximowiczii和S. pulchella。结论本文开发的SSR标记将对今后对多裂链球菌及相关物种的种群遗传学研究有用。

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