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首页> 外文期刊>Applications in plant sciences. >Twenty-Seven Low-Copy Nuclear Primers for Lindera obtusiloba (Lauraceae): A Tertiary Relict Species in East Asia
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Twenty-Seven Low-Copy Nuclear Primers for Lindera obtusiloba (Lauraceae): A Tertiary Relict Species in East Asia

机译：obLindera obtusiloba（Lauraceae）的二十七个低拷贝核引物：东亚第三纪的遗留物

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摘要

Premise of the study: To investigate a more detailed evolutionary history of Lindera obtusiloba (Lauraceae) and other Lindera species, polymorphic low-copy nuclear primers were developed. Methods and Results: Unigenes of the L. obtusiloba transcriptome greater than 800 bp in length were randomly chosen for initial design of 168 primers. Agarose gel electrophoresis and Sanger sequencing were used to select low-copy nuclear genes. Twenty-seven primers were obtained and were used to investigate genetic diversity in 90 individuals from 24 populations. The nucleotide diversity ranged from 2.11 × 10-3 to 8.99 × 10-3, and haplotype diversity ranged from 0.57 to 0.97. These primers were also cross-amplified in L. aggregata, L. chunii, L. erythrocarpa, and L. glauca ; up to 15 primers were successfully amplified in these related species. Conclusions: This methodology is effective for development of low-copy nuclear primers. The 27 primers developed here will be useful for evolutionary studies of L. obtusiloba and other Lindera species.
机译：研究的前提：为了研究更详细的Lindera obtusiloba（Lauraceae）和其他Lindera物种的进化史，开发了多态性低拷贝核引物。方法和结果：随机选择长度大于800 bp的钝角乳杆菌转录组的单基因，以初步设计168个引物。琼脂糖凝胶电泳和Sanger测序被用于选择低拷贝核基因。获得了27个引物，用于研究24个种群中90个个体的遗传多样性。核苷酸多样性范围为2.11×10 -3 至8.99×10 -3 ，单倍型多样性范围为0.57至0.97。这些引物也在聚合乳杆菌，矮牵牛乳，红皮乳杆菌和青冈乳杆菌中交叉扩增。在这些相关物种中成功扩增了多达15个引物。结论：该方法对于低拷贝核引物的开发是有效的。此处开发的27个引物将对Obtusiloba和其他Lindera物种的进化研究有用。

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