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首页> 外文期刊>Applications in plant sciences. >In silico detection of polymorphic microsatellites in the endangered Isis tamarind, Alectryon ramiflorus (Sapindaceae)
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In silico detection of polymorphic microsatellites in the endangered Isis tamarind, Alectryon ramiflorus (Sapindaceae)

机译：在计算机上对濒临灭绝的伊希斯罗望子罗望子，电伊豆（Sapindaceae）中的多态微卫星进行计算机检测

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Premise of the Study Alectryon ramiflorus (Sapindaceae) is an endangered rainforest tree known from only two populations. In this study, we identified polymorphic microsatellites, in silico, improving the effectiveness and efficiency of microsatellite development of nonmodel species. The development of genetic markers will support future conservation management of the species. Methods and Results We used next‐generation sequencing and bioinformatics to detect polymorphic microsatellites, in silico, reducing both the time and cost of marker development. A panel of 15 microsatellites, 12 of which were polymorphic, were subsequently characterized in 64 adult trees representing the entire species range. Mean observed heterozygosity and expected heterozygosity were 0.471 and 0.425, respectively. The polymorphism information content across loci ranged from 0.152 to 0.875. Conclusions The microsatellite markers developed in this study will be useful in gaining an understanding of A. ramiflorus ’ genetic diversity, level of inbreeding, and population structure and for guiding future restoration and management efforts.
机译：研究前提Alectryon ramiflorus（Sapindaceae）是一种濒临灭绝的雨林树，仅从两个种群中得知。在这项研究中，我们确定了计算机上的多态微卫星，从而提高了非模型物种开发微卫星的有效性和效率。遗传标记的发展将支持该物种的未来保护管理。方法和结果我们使用了下一代测序和生物信息学技术来检测计算机多态性微卫星，从而减少了标记开发的时间和成本。随后在代表整个物种范围的64棵成年树木中鉴定了一组15个微卫星，其中12个是多态的。平均观察到的杂合度和预期杂合度分别为0.471和0.425。整个基因座的多态性信息含量范围为0.152至0.875。结论本研究中开发的微卫星标记将有助于了解A. ramiflorus的遗传多样性，近交水平和种群结构，并指导未来的恢复和管理工作。

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