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首页> 外文期刊>Advancements in Life Sciences >Genetic diversity and phylogenetic relationship in different genotypes of cotton for future breeding
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Genetic diversity and phylogenetic relationship in different genotypes of cotton for future breeding

机译：不同基因型棉花的遗传多样性和系统进化关系

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Background: To make the plants well adapted and more resistant to diseases and other environmental stresses there is always a need to improve the quality of plant’s genome i.e. to increase its genetic diversity. Methods: In the present study six variety and six lines of cotton were investigated for their genetic diversity and phylogenetic relationship. For this purpose 35 different RAPD primers obtained from the Gene Link Technologies, USA were used. Results: Among 35 RAPD primers, 13 primers produced reproducible PCR bands while the rest failed to show any amplification product. Our results indicated that the total count of the reproducible bands was 670 and polymorphic loci were counted to be 442 which constitute 66% of total loci. Phylogenetic analysis revealed two major groups each consists of 7 and 5 genotypes respectively. Genotypes Lp1 and Tp4 were placed at maximum genetic distance and in separate groups and could be utilized for future cotton breeding. Conclusions: RAPD analysis is a cheaper and time saving technique for the determination of genetic diversity of different cotton genotypes. Cotton genotype Lp1 and Tp4 could be the best candidates for future breeding programs as both genotypes are genetically distant from each other.
机译：背景：为了使植物适应性强，更能抵抗疾病和其他环境压力，始终需要提高植物基因组的质量，即增加其遗传多样性。方法：在本研究中，研究了六个品种和六个品系的棉花的遗传多样性和系统发育关系。为此，使用了35种不同的从美国Gene Link Technologies获得的RAPD引物。结果：在35条RAPD引物中，有13条引物产生可重复的PCR条带，其余引物未显示任何扩增产物。我们的结果表明，可复制条带的总数为670，多态性位点计为442，占总位点的66％。系统发育分析表明，两个主要类别分别由7和5个基因型组成。基因型Lp1和Tp4处于最大遗传距离且位于单独的组中，可用于将来的棉花育种。结论：RAPD分析是一种便宜，省时的技术，可用于确定不同基因型棉花的遗传多样性。棉花基因型Lp1和Tp4可能是未来育种计划的最佳候选者，因为这两个基因型在遗传上都相距遥远。

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