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Sequence Diversity of MAOA Gene within Wild and Docile Animal Species

机译：野生和驯养动物物种中MAOA基因的序列多样性

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Background: Molecular characterization of MAOA gene was performed to investigate aggressivebehaviour within wild (lion, leopard, and wolf) as opposed to docile animal (sheep, goat) speciesliving in different habitats, by undertaking sequence diversity analysis of this gene.Methods: The MAOA gene was partially amplified by PCR for wild and docile animal species. AmplifiedDNA was sequenced and then analyzed using BioEdit and Sequencher softwares, while multiple sequencealignment and phylogenetics analysis were conducted through MEGA software. Bioinformatics tool likeProsite scan, Motif Scan and Prot Param were used to study properties of mutant proteins of MAOA gene.Results: Different polymorphic sites were observed which included c.956, c.1063 in docile animals andc.2530 in wild animals. Phylogenetic analysis based on this candidate gene endorsed the existing taxonomyof subject animals, while bioinformatics tools explored the altered characteristics of mutantprotein.Conclusion: The newly found polymorphic loci in wild and docile animals in this study could have a rolein behavioral response and acclimatization within their peculiar habitats. This study also highlights thegenetic diversity of MAOA gene, which will add knowledge to the existing animal genetic resource ofPakistan.
机译：背景：通过对MAOA基因进行序列多样性分析，对MAOA基因进行分子表征，以研究野生（狮子，豹和狼）与生活在不同栖息地的温顺动物（绵羊，山羊）物种之间的攻击行为。通过PCR对野生和温顺动物物种部分扩增了该基因。对扩增的DNA进行测序，然后使用BioEdit和Sequencher软件进行分析，同时通过MEGA软件进行多重序列比对和系统进化分析。通过Prosite scan，Motif Scan和Prot Param等生物信息学工具对MAOA基因突变蛋白的性质进行了研究。结果：观察到了多态性位点，其中温顺动物的c.956，c.1063和野生动物的c.2530。基于该候选基因的系统发育分析证实了对象动物的现有分类学，而生物信息学工具探索了突变蛋白的改变特征。结论：本研究中新发现的野生和温顺动物多态性位点可能在其行为反应和适应环境中起作用栖息地。这项研究还强调了MAOA基因的遗传多样性，这将为巴基斯坦现有的动物遗传资源增加知识。

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