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首页> 外文期刊>Advancements in Life Sciences >Arabidopsis Argininosuccinate Lyase and Argininosuccinate Synthase are important for resistance against Pseudomonas syringae
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Arabidopsis Argininosuccinate Lyase and Argininosuccinate Synthase are important for resistance against Pseudomonas syringae

机译：拟南芥精氨酸琥珀酸裂合酶和精氨酸琥珀酸合酶对于抵抗丁香假单胞菌很重要

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Background: Arginine is one of the important amino acids and is involved in a variety of plant responses against biotic and abiotic stresses. Two important genes Argininosuccinate Synthase ( AS) and Argininosuccinate Lyase ( AL ) are associated with the production of arginine from ornithine. Here we report molecular characterization of Arabidopsis thaliana artificial micro RNA (amiRNA) mutants for the genes AL and AS in response to infection with Pseudomonas syringae DC3000. Methods: Quantitative real time PCR (qRT-PCR) and Promoter::GUS plant lines were used for expression analysis. Artificial micro RNAs (amiRNA) mediated gene silencing was used to generate mutant plant lines. Flood inoculation technique was used for infection test essays with Pseudomonas syringae . Results: Expression analysis of A. thaliana plants harboring promoter AS ::GUS construct showed strong promoter activity upon P. syringae infection. Quantitative real time PCR (qRT-PCR) analysis showed that AL and AS expression was strongly induced upon infection with P. syringae . Infection essays for P. syringae showed enhanced susceptibility to virulent (Pto) as well as avirulent (?avr Pto /?avr Pto B) strains of P. syringae . However, mutant plants infiltered with infiltration medium containing 1 mM L-arginine regain their resistance in comparison with wild type (Col-0) plants. Conclusion: Our findings suggest that genes related to arginine metabolism play a key role in plant defenses during P. syringae infection on A. thaliana . This study revealed that proper functioning of arginine related genes is required to deploy defense response against P. syringae. Decrease in the expression of these genes improves conditions for the growth of pathogen.
机译：背景：精氨酸是重要的氨基酸之一，参与多种植物应对生物和非生物胁迫的反应。两个重要的基因精氨酸琥珀酸合酶（AS）和精氨酸琥珀酸裂合酶（AL）与从鸟氨酸生产精氨酸有关。在这里，我们报告拟南芥人工微RNA（amiRNA）突变体的基因AL和AS响应丁香假单胞菌DC3000感染的分子表征。方法：采用实时定量PCR（qRT-PCR）和Promoter :: GUS植物品系进行表达分析。人工微RNA（amiRNA）介导的基因沉默被用来产生突变植物株。采用洪水接种技术进行丁香假单胞菌的感染试验。结果：带有启动子AS :: GUS构建体的拟南芥植物的表达分析显示在丁香假单胞菌感染后具有强启动子活性。实时荧光定量PCR（qRT-PCR）分析表明，丁香假单胞菌感染后可强烈诱导AL和AS表达。丁香假单胞菌的感染文章显示对丁香假​​单胞菌的毒力（Pto）和无毒（ΔavrPto /ΔavrPto B）菌株的敏感性增强。然而，与野生型（Col-0）植物相比，用含有1mM L-精氨酸的浸润培养基浸润的突变植物恢复了其抗性。结论：我们的发现表明，与精氨酸代谢相关的基因在拟南芥中丁香假单胞菌感染期间在植物防御中起关键作用。这项研究表明精氨酸相关基因的适当功能是部署针对丁香假单胞菌的防御反应所必需的。这些基因表达的减少改善了病原体生长的条件。

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