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首页> 外文期刊>Aquaculture and Fisheries >Cloning of a microphthalmia-associated transcription factor gene and its functional analysis in nacre formation and melanin synthesis in Hyriopsis cumingii
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Cloning of a microphthalmia-associated transcription factor gene and its functional analysis in nacre formation and melanin synthesis in Hyriopsis cumingii

机译：小眼球菌相关转录因子基因的克隆及其在三角帆蚌中珍珠层形成和黑色素合成中的功能分析

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摘要

Microphthalmia-associated transcription factor (MITF) is an essential transactivator in melanin synthesis. To characterize the role of MITF in the pearl mussel Hyriopsis cumingii , the MITF homolog of H. cumingii was isolated. The full-length HcMitf cDNA consisted of a 1332-bp with an open reading frame that encode for a 443 amino acid protein that contain a conserved basic helix-loop-helix zipper domain. The HcMitf was found to widespread tissue distribution but expression was higher in purple mussels than in white mussels, mostly in mantle, liver, kidney, gill, and foot with the exception of the adductor mussel. HcMitf and its downstream gene tyrosinase ( HcTyr ) were highly expressed at the nacre deposition stage after implantation of mantle tissue to produce pearls. Using RNA interference, the expression of HcMitf was reduced by 78% ( P ??0.01) and expression of HcTyr was also significantly suppressed and consequently total melanin content was decreased ( P ??0.05). The results suggest that HcMitf plays an important role in melanin synthesis, nacre formation and shell pigmentation in the H. cumingii .
机译：小眼症相关转录因子（MITF）是黑色素合成中必不可少的反式激活因子。为表征MITF在珍珠贻贝三角帆蚌中的作用，分离了H. cumingii的MITF同源物。全长HcMitf cDNA由一个1332 bp的开放阅读框组成，该阅读框编码443个氨基酸的蛋白质，其中包含一个保守的基本螺旋-环-螺旋拉链结构域。 HcMitf被发现分布在广泛的组织中，但是紫色贻贝中的表达高于白色贻贝，除了内收肌以外，大部分在地幔，肝脏，肾脏，g和足中表达。 HcMitf及其下游基因酪氨酸酶（HcTyr）在植入地幔组织以产生珍珠后的珍珠质沉积阶段高表达。使用RNA干扰，HcMitf的表达降低了78％（P 0.01），HcTyr的表达也得到了显着抑制，因此总黑色素含量降低了（P 0.05）。结果表明，HcMitf在H. cumingii的黑色素合成，珍珠层形成和壳色素沉着中起重要作用。

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