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首页> 外文期刊>Advancements in Life Sciences >Hemoglobin adducts in paint industry workers: An electrophoretic analysis
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Hemoglobin adducts in paint industry workers: An electrophoretic analysis

机译：涂料行业工人中的血红蛋白加合物：电泳分析

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Background: Hemoglobin (Hb) has a significant role among other blood proteins vital forcarrying nutrients to blood cells. Being a conjugated protein, Hb is prone to be captured bycompounds of low molecular weight like organic acid anhydrides (OAAs) which are prominentindustrial/occupational hazards. Hindered or lowered availability of Hb to blood cells can causeanemia, thalassemia and porphyria. Along with these disorders, workers exposed to OAAs canalso acquire like type-I allergy, type-IV allergy, skin problems, rhinitis and asthma. Revelationof Hb-OAAs compounds prior to appearance of actual symptoms could be important forsubsequent therapy.Method: The Hb separation was achieved successfully by SDS-PAGE electrophoresis on 10-15% gels of different concentration, stained with CBB-R250 Blue. Total of 66 blood proteinsamples were used for the comparative study of exposed workers of paint industry workerswith control (normal) group to detect proteins, which might serve as marker for the early diseasediagnosis.Results: The better Hb separation resolution was achieved on 12% gel as shown inelectrograms. The electrograms of paint workers exposed to OAAs showed bands at 12, 48, 66,78, 128 and 132 KDa in most of cases. In normal cases the bands were found at 13, 30, 48, 67,76, 125 and 155 KDa in majority of control samples for Hb electrophoresis.Conclusion: This study supports the association between Hb and OAAs adducts among theexposed paint workers from hypersensitive effects like fever (rhinitis) leading to asthma, skinallergies and major clinical effects.
机译：背景：血红蛋白（Hb）在将营养物质输送到血细胞中至关重要的其他血液蛋白质中起着重要作用。作为结合蛋白，Hb容易被低分子量的化合物（如有机酸酐（OAA））捕获，这是工业/职业危害的主要来源。血细胞中Hb的可用性受阻或降低会导致贫血，地中海贫血和卟啉症。除这些疾病外，接触OAA的工人还可能获得I型过敏，IV型过敏，皮肤问题，鼻炎和哮喘。方法：在10-15％不同浓度的CBB-R250蓝染色的凝胶上，通过SDS-PAGE电泳成功地完成了Hb的分离。总共66份血液蛋白样品用于对照组（正常）的涂料行业裸露工人的比较研究，以检测蛋白，这些蛋白可以作为早期疾病诊断的标志物。结果：在12％的凝胶上可获得更好的Hb分离分辨率如电图所示。在大多数情况下，暴露于OAA的油漆工人的电描记图显示出12、48、66、78、128和132 KDa的谱带。在正常情况下，大多数Hb电泳对照样品的谱带在13、30、48、67、76、125和155 KDa处发现。结论：本研究支持暴露的油漆工人中Hb和OAAs加合物之间存在超敏效应。如发烧（鼻炎）会导致哮喘，皮肤过敏和重大临床影响。

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