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首页> 外文期刊>Archives of Biological Sciences >ANKRD1-MEDIATED SIGNALING IS SUPPORTED BY ITS INTERACTION WITH ZONULA OCCLUDENS-1
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ANKRD1-MEDIATED SIGNALING IS SUPPORTED BY ITS INTERACTION WITH ZONULA OCCLUDENS-1

机译：ANKRD1介导的信号与ZONULA OCCLUDENS-1的相互作用得到支持

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The muscle ankyrin repeat protein Ankrd1 is localized in a mechanosensory complex of the sarcomeric I-band. It is involved in signaling pathways activated in response to mechanical stretch. It also acts as a transcriptional cofactor in the nucleus, playing an important role in cardiogenesis and skeletal muscle differentiation. To investigate its regulatory function in signaling we employed protein array methodology and identified 10 novel Ankrd1 binding partners among PDZ domain proteins known to act as platforms for multiprotein complex assembly. The zonula occludens protein-1 (ZO-1) was chosen for further analysis since its interaction with Ankrd2 had already been demonstrated. Both Ankrd2 and Ankrd1 have similar functions and localize in the same regions. We confirmed the interaction of Ankrd1 with ZO-1 protein and determined their subcellular distribution in HeLa cells, showing their colocalization in the cytoplasm. Our findings corroborate the role of Ankrd1 in intracellular signaling.
机译：肌肉锚蛋白重复蛋白Ankrd1位于肌节I带的机械感官复合体中。它参与响应机械拉伸而激活的信号传导途径。它还在细胞核中起转录辅助因子的作用，在心脏发生和骨骼肌分化中起重要作用。为了研究其在信号传导中的调控功能，我们采用了蛋白质阵列方法，并在PDZ域蛋白中鉴定出10个新颖的Ankrd1结合伴侣，这些蛋白被称为多蛋白复合物组装平台。选择小带闭合蛋白-1（ZO-1）进行进一步分析，因为已经证明了其与Ankrd2的相互作用。 Ankrd2和Ankrd1都具有相似的功能，并且位于相同的区域。我们证实了Ankrd1与ZO-1蛋白的相互作用，并确定了它们在HeLa细胞中的亚细胞分布，显示了它们在细胞质中的共定位。我们的发现证实了Ankrd1在细胞内信号传导中的作用。

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