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首页> 外文期刊>Archives of Biological Sciences >EXPRESSION OF MAJOR ECTONUCLEOTIDASES AFTER CORTICAL STAB BRAIN INJURY IN RATS: A REAL-TIME PCR STUDY
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EXPRESSION OF MAJOR ECTONUCLEOTIDASES AFTER CORTICAL STAB BRAIN INJURY IN RATS: A REAL-TIME PCR STUDY

机译：大鼠皮层硬脑膜损伤后主要核酸酶的表达：实时PCR研究

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Ectonucleotidases are cell surface-located enzymes responsible for the extracellular degradation of nucleotides. They are comprised of several protein families: ectonucleoside triphosphate diphosphohydrolases (E-NTPDase), ectonucleotide pyrophosphatase/phosphodiesterases (E-NPPases) and ecto-5’-nucleotidase. Previously we showed that cortical stab injury alters ectonucleotidase activities in the rat brain, but that the specific enzymes responsible for these changes were not identified. In this study we investigated the gene expression of the specific ectonucleotidase enzymes, NTPDase1-3, NPP1-3 and ecto-5’-nucleotidase, two and seven days after cortical stab injury in rats, using real-time PCR. Two days after the injury we observed only one significant change: the downregulation in NTPDase2 mRNA expression. Our results indicate that traumatic brain injury induces significant upregulation of NTPDase1, NTPDase2 and ecto-5’-nucleotidase transcripts, and the downregulation of NPP1, seven days after the injury. Thus, traumatic brain injury has diverse impacts on ectonucleotidases gene expression, which may be reflected in the enzyme activities and extracellular nucleotide concentrations in the perilesional tissue.
机译：外源核苷酸是细胞表面定位的酶，负责核苷酸的细胞外降解。它们由几个蛋白质家族组成：外核苷三磷酸二磷酸水解酶（E-NTPDase），外核苷酸焦磷酸酶/磷酸二酯酶（E-NPPases）和ecto-5’-核苷酸酶。先前，我们显示皮质刺伤可改变大鼠大脑中的外切核苷酸酶活性，但尚未鉴定出负责这些变化的特定酶。在这项研究中，我们使用实时荧光定量PCR技术研究了大鼠皮层刺伤后两天和七天，特定的外切核苷酸酶NTPDase1-3，NPP1-3和ecto-5'核苷酸酶的基因表达。受伤两天后，我们仅观察到一个显着变化：NTPDase2 mRNA表达下调。我们的结果表明，颅脑损伤在损伤后7天会诱导NTPDase1，NTPDase2和ecto-5′-核苷酸酶转录本的显着上调，以及NPP1的下调。因此，脑外伤对外切核苷酸酶基因表达具有多种影响，这可能反映在病灶周围组织中的酶活性和细胞外核苷酸浓度上。

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