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首页> 外文期刊>Archives of clinical infectious diseases. >Prevalence, Molecular Diversity, and Antimicrobial Resistance Patterns of Pathogenic Bacteria Isolated From Medical Foods, Food Staff, Cooking Instruments, and Clinical Samples in a Teaching Hospital in Tehran, Iran
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Prevalence, Molecular Diversity, and Antimicrobial Resistance Patterns of Pathogenic Bacteria Isolated From Medical Foods, Food Staff, Cooking Instruments, and Clinical Samples in a Teaching Hospital in Tehran, Iran

机译：从伊朗德黑兰的一家教学医院的医用食品，食品人员，烹饪用具和临床样品中分离出的致病细菌的患病率，分子多样性和抗药性模式

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Background: Medical foods could be vehicles of pathogenic microbes for vulnerable people in the hospitals. Hospital kitchen is considered as the main source of this cross-contamination. Objectives: The current study aimed at investigating the frequency of bacterial species and their antimicrobial resistance patterns in foods, food handlers, and utensils compared with those of the clinical isolates in a hospital kitchen in Tehran, Iran. Methods: This cross sectional study was performed in a hospital in Tehran, Iran from April 2011 to January 2013. Accordingly, simple random sampling of raw and cooked food materials, swab samples of cooking utensils, and hands and noses of food staff were done. Clinical samples were collected from blood, urine, wound, and respiratory aspirates of patients with hospital acquired infections. Bacterial isolates were identified according to biochemical standard identification schema. Antimicrobial susceptibility of the strains was determined by disk diffusion method according to the CLSI (the clinical and laboratory standards institute) guidelines. Molecular diversity of indicator bacterial isolates of Staphylococcus aureus and Escherichia coli in the kitchen and those of the isolated ones from intensive care unit were also investigated by molecular typing method. The occurrence of cross-contamination was hypothesized based on the results of phylogenetic investigation and resistance biotyping. Results: Out of the 200 kitchen samples, S. aureus , E. coli , Acinetobacter spp., Pseudomonas spp., and Enterococcus spp. were isolated in frequencies of 15.5%, 8%, 2.5%, 0.5%, and 0.5%, respectively. Prevalence of multidrug resistant-methicillin resistant strains of S. aureus (MDR-MRSA) in the samples of the hospital kitchen vs the intensive care unit (ICU) was 18.7% (6/32), compared with 91.6% (22/24), respectively. Among the kitchen E. coli isolates, MDR pattern was detected in a frequency of 52.9%; the highest frequency was detected among the isolates of utensils. Although the results of the phylogenetic and resistance biotyping analyses did not confirm significant relationship between the isolates of the ICU and hospital kitchen, this similarity was confirmed among the strains isolated from the foods, food handlers, and utensils. In this regard, food staff and utensils were considered as the main sources of cross-contamination for S. aureus and E. coli , respectively. Conclusions: Weak health conditions of food workers and inadequate cooking to eliminate the contaminants during food processing were postulated as the main risk factors for transmission of these bacteria, through medical foods. into hospital.
机译：背景：医疗食品可能是医院中易受伤害人群的病原微生物媒介。医院厨房被认为是这种交叉污染的主要来源。目的：目前的研究旨在调查与伊朗德黑兰一家医院厨房中的临床分离菌相比，食品，食物处理者和器皿中细菌种类的频率及其抗药性模式。方法：这项横断面研究于2011年4月至2013年1月在伊朗德黑兰的一家医院中进行。因此，对生食和熟食原料，拭子样本以及炊事人员的鼻子和手进行了简单的随机抽样。从医院获得性感染患者的血液，尿液，伤口和呼吸道中收集临床样本。根据生化标准鉴定方案鉴定细菌分离株。根据CLSI（临床和实验室标准协会）指南，通过圆盘扩散法确定了菌株的抗菌敏感性。还通过分子分型方法研究了厨房中金黄色葡萄球菌和大肠埃希菌的指示细菌分离株以及重症监护室分离出的细菌的分子多样性。根据系统发育研究和抗性生物分型的结果，假设发生了交叉污染。结果：在200个厨房样品中，金黄色葡萄球菌，大肠杆菌，不动杆菌属，假单胞菌属和肠球菌属。隔离的频率分别为15.5％，8％，2.5％，0.5％和0.5％。医院厨房与重症监护室（ICU）样本中金黄色葡萄球菌（MDR-MRSA）的多药耐药性耐甲氧西林菌株（MDR-MRSA）的发生率为18.7％（6/32），而91.6％（22/24） ， 分别。在厨用大肠埃希菌中，检出MDR的频率为52.9％。在餐具的分离物中检出的频率最高。尽管系统发育和耐药性生物型分析的结果并未确认ICU分离株与医院厨房之间的显着相关性，但在从食品，食品处理者和用具中分离出的菌株之间也证实了这种相似性。在这方面，食品人员和餐具分别被认为是金黄色葡萄球菌和大肠杆菌交叉污染的主要来源。结论：假定食品工人的健康状况较弱以及在食品加工过程中烹饪不足以消除污染物，被认为是通过医疗食品传播这些细菌的主要危险因素。进医院。

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