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首页> 外文期刊>Archives of clinical infectious diseases. >High Frequency of icaAD , clumping factors A/B, fib and eno Genes in Staphylococcus aureus Species Isolated From Wounds in Tehran, Iran during 2012-2013
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High Frequency of icaAD , clumping factors A/B, fib and eno Genes in Staphylococcus aureus Species Isolated From Wounds in Tehran, Iran during 2012-2013

机译：2012-2013年伊朗德黑兰从伤口分离的金黄色葡萄球菌物种中icaAD，聚集因子A / B，fib和eno基因的高频率

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Background: Staphylococcus aureus ( S. aureus ) isolates have emerged in healthcare and community, and cause a wide spectrum of clinical signs. On the other hand, methicilin-resistant S. aureus (MRSA) isolates can resist a wide range of antibiotics, which can make treatment of infections much more difficult. Objectives: The current study aimed to determine S. aureus characteristics isolated from wounds. Materials and Methods: This definitive and cross-sectional study was conducted in Tehran, Iran. A total of 15 S. aureus isolates were collected from wound samples with sterile swabs and were identified by the conventional diagnostic tests. The antibiotic susceptibility pattern was performed according to the Clinical and Laboratory Standards Institute (CLSI, 2012) protocol. The mecA gene, Staphylococcal Cassette Chromosome mec (SCC mec ) types, agr specific groups and biofilm related genes were detected by Polymerase Chain Reaction (PCR) assay. Results: The majority of the isolates were resistant to amoxicillin, tetracycline and erythromycin, but all were susceptible to vancomycin and linezolid. Eight (40%) isolates were methicillin resistant and the mecA gene was detected in these isolates. The majority of (90%) of MRSA harbored SCC mec type III, and two isolates harbored type V. The majority (70%, n = 14) of the isolates belonged to agr I, followed by agr II (15%, n = 3), agr IV (10%, n = 2) and agr III (5%, n = 1). The frequencies of clfAB , fnbAB , fib , eno , cna , ebps and bbp genes were 100%, 100%, 65% (n = 13), 55% (n = 11), 70% (n = 14), 70% (n = 14), 55% (n = 11), 0% and 0%, respectively. Conclusions: Half of the isolates were MRSA, the majority of which harbored SCC mec type III. Moreover, most of S. aureus isolates belonged to agr I. The frequency of icaAD , clfAB , fib and eno genes were high in S. aureus species isolated from wounds.
机译：背景：金黄色葡萄球菌（金黄色葡萄球菌）分离株已出现在医疗保健和社区中，并引起广泛的临床体征。另一方面，耐甲氧西林的金黄色葡萄球菌（MRSA）分离株可以抵抗多种抗生素，这会使感染的治疗更加困难。目的：目前的研究旨在确定从伤口分离出的金黄色葡萄球菌的特征。材料和方法：这项确定性的横断面研究在伊朗德黑兰进行。用无菌拭子从伤口样本中收集了总共15株金黄色葡萄球菌，并通过常规诊断测试进行了鉴定。抗生素敏感性模式是根据临床和实验室标准协会（CLSI，2012）方案进行的。通过聚合酶链反应（PCR）法检测了mecA基因，葡萄球菌盒式染色体mec（SCC mec）类型，agr特异性基团和生物膜相关基因。结果：大多数分离株对阿莫西林，四环素和红霉素有抗性，但都对万古霉素和利奈唑胺敏感。八株（40％）菌株对甲氧西林具有抗性，并且在这些菌株中检测到mecA基因。 MRSA的大多数（90％）具有SCC mec III型，两个分离株具有V型。大多数（70％，n = 14）分离株属于agr I，其次是agr II（15％，n = 3），agr IV（10％，n = 2）和agr III（5％，n = 1）。 clfAB，fnbAB，fib，eno，cna，ebps和bbp基因的频率分别为100％，100％，65％（n = 13），55％（n = 11），70％（n = 14），70％ （n = 14），55％（n = 11），0％和0％。结论：一半的分离株是MRSA，其中大多数带有SCC mec III型。此外，大多数金黄色葡萄球菌分离物都属于agrI。从伤口分离的金黄色葡萄球菌物种中icaAD，clfAB，fib和eno基因的频率很高。

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