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首页> 外文期刊>Artificial cells, nanomedicine, and biotechnology. >Use new poly (ε-caprolactone/collagen/NBG) nerve conduits along with NGF for promoting peripheral (sciatic) nerve regeneration in a rat
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Use new poly (ε-caprolactone/collagen/NBG) nerve conduits along with NGF for promoting peripheral (sciatic) nerve regeneration in a rat

机译：使用新的聚（ε-己内酯/胶原/ NBG）神经导管和NGF促进大鼠周围神经（坐骨神经）再生

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Regeneration of peripheral nerve defects remained a remarkable clinical challenge. Engineered nerve conduits represent a promising strategy to improve functional recovery in peripheral nerve injury repair. However, nerve conduits require additional factors such as neurotrophic factors to create a more conducive microenvironment for nerve regeneration. Neurotrophic factors have well-demonstrated abilities to improve neurite outgrowth, making them great candidates for repairing of defected nerves. To this end, we examined the beneficial effects of repairing the transected rat sciatic nerve by loading of nerve growth factor (NGF) in nerve conduits. The PCL/Collagen/NBG conduits were interposed into the 10?mm right sciatic nerve defects. Twenty-four rats were randomly allocated into four groups: 1- nerve autograft group, 2- a nongrafted group with gap 10-mm, 3- conduit group and 4- the conduits loaded with NGF. Motor and sensory functional recovery, the evoked muscle action potential, and motor distal latency showed significant improvement in rats treated with NGF. The histology and immunohistochemistry studies revealed less fibrosis and a high level of expression of CD31 and NF-200 protein at the crush site in the Conduit?+?NGF group. In conclusion, the PCL/Collagen/NBG conduit loaded with NGF, which exhibited nanometer-scale features, neurotrophic activity, favorable mechanical properties and biocompatibility could improve sciatic nerve regeneration in rats.
机译：周围神经缺损的再生仍然是一项重大的临床挑战。工程化的神经导管代表了一种有前途的策略，可以改善周围神经损伤修复中的功能恢复。但是，神经导管需要其他因素（例如神经营养因子）来创建更有利于神经再生的微环境。神经营养因子具有改善神经突生长的良好能力，使其成为修复缺损神经的理想人选。为此，我们研究了通过在神经导管中加载神经生长因子（NGF）来修复横断大鼠坐骨神经的有益效果。将PCL /胶原蛋白/ NBG导管插入10mm的右坐骨神经缺损中。 24只大鼠随机分为四组：1-神经自体移植组，2-神经间隙为10mm的非移植组，3-导管组和4-装有NGF的导管。运动和感觉功能恢复，诱发的肌肉动作电位以及运动末梢潜伏期在用NGF治疗的大鼠中显示出明显的改善。组织学和免疫组织化学研究显示，Conduit？+？NGF组的挤压部位纤维化程度较低，CD31和NF-200蛋白的表达水平较高。总之，载有NGF的PCL / Collagen / NBG导管具有纳米级特征，神经营养活性，良好的机械性能和生物相容性，可改善大鼠坐骨神经的再生。

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