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首页> 外文期刊>Artificial cells, nanomedicine, and biotechnology. >Comparison of protein- and polysaccharide-based nanoparticles for cancer therapy: synthesis, characterization, drug release, and interaction with a breast cancer cell line
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Comparison of protein- and polysaccharide-based nanoparticles for cancer therapy: synthesis, characterization, drug release, and interaction with a breast cancer cell line

机译：比较基于蛋白质和多糖的纳米颗粒用于癌症治疗：合成，表征，药物释放以及与乳腺癌细胞系的相互作用

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Abstract In this study, human serum albumin (HSA) was used as a protein-based material and poly (3-hydroxybutyrate) (PHB)–carboxymethyl chitosan (CMCh) as a polysaccharide-based material for the production of nanoparticles to be used as nanocarriers in cancer therapy. HSA and PHB–CMCh nanoparticles were prepared and characterized with a Zeta Sizer, Fourier transform infrared spectroscopy, scanning electron microscopy, and atomic force microscope. The effects of the pH value of the suspending medium and the amounts of crosslinker and polymer concentration on nanoparticle size and size distribution were investigated. The anticancer-agent etoposide was used as a model drug and encapsulated in nanoparticles to obtain drug release profiles. The entrapment efficiency of HSA nanoparticles was found to be greater than that of PHB–CMCh nanoparticles. To achieve “active” targeting of cancer cells, the nanoparticles were modified with concanavalin A. In the final step of the study, the interaction of nanoparticles with cancer cells was investigated in cytotoxicity and cellular uptake studies.
机译：摘要在本研究中，人血清白蛋白（HSA）被用作蛋白质基材料，而聚（3-羟基丁酸酯）（PHB）-羧甲基壳聚糖（CMCh）被用作多糖基材料，用于制备纳米颗粒。癌症治疗中的纳米载体。制备了HSA和PHB–CMCh纳米颗粒，并使用Zeta Sizer，傅立叶变换红外光谱，扫描电子显微镜和原子力显微镜对其进行了表征。研究了悬浮介质的pH值，交联剂的量和聚合物浓度对纳米颗粒尺寸和尺寸分布的影响。将抗癌药依托泊苷用作模型药物，并封装在纳米颗粒中以获得药物释放曲线。发现HSA纳米颗粒的包封效率高于PHB–CMCh纳米颗粒的包封效率。为了实现癌细胞的“活性”靶向，用伴刀豆球蛋白A修饰了纳米颗粒。在研究的最后一步，在细胞毒性和细胞摄取研究中研究了纳米颗粒与癌细胞的相互作用。

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