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首页> 外文期刊>Artificial cells, nanomedicine, and biotechnology. >Comparison of some biochemical properties of artichoke polyphenol oxidase entrapped in alginate-carrageenan and alginate gels
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Comparison of some biochemical properties of artichoke polyphenol oxidase entrapped in alginate-carrageenan and alginate gels

机译：海藻酸盐-角叉菜胶和海藻酸盐凝胶中捕获的朝鲜蓟多酚氧化酶的某些生化特性比较

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摘要

Polyphenol oxidase (PPO, EC.1.14.18.1) isolated from artichoke (Cynara scolymus) was entrapped within alginate and alginate+ carrageenan beads, and the catecholase and cresolase activities of both entrapped enzymes were determined. Some properties of these immobilized enzymes such as optimum pH and temperature, kinetic parameters (Km and Vmax), thermal, and storage stability were determined and compared to each other.The highest catecholase activity was observed in alginate gel (370 U/g bead) while the highest cresolase activity was in alginate+ carrageenan gel (90 U/g bead). For catecholase and cresolase activities, optimum pHs of alginate and alginate+ carrageenan beads were determined to be 7.0 and 4.0, respectively. Optimum temperatures for catecholase activity were determined to be 40°C for both entrapped enzymes. These values for cresolase activity were 30°C and 20°C, respectively. Immobilized artichoke PPOs greatly preserved their thermal stability which exists anyway. The catalytic efficiency value (Vmax/Km) of the alginate beads is approximately high as two-and-a-half folds of that of alginate+κ-carrageenan beads for cresolase activity. These values were very close for catecholase activity. Immobilized beads saved their both activities after 30 days of storage at 4°C.
机译：从洋蓟（Cynara scolymus）分离出的多酚氧化酶（PPO，EC.1.14.18.1）被捕获在藻酸盐和藻酸盐+角叉菜胶珠中，并测定了两种被捕获酶的儿茶酚酶和甲酚酶活性。确定并比较了这些固定化酶的一些特性，例如最佳pH和温度，动力学参数（Km和Vmax），热稳定性和储存稳定性。在藻酸盐凝胶中观察到最高的儿茶酚酶活性（370 U / g珠）而最高的甲酚酶活性是藻酸盐+角叉菜胶（90 U / g珠）。对于儿茶酚酶和甲酚酶活性，确定藻酸盐和藻酸盐+角叉菜胶珠的最佳pH分别为7.0和4.0。两种包埋的酶的最佳儿茶酚酶活性温度确定为40°C。甲酚酶活性的这些值分别是30℃和20℃。固定的朝鲜蓟PPO极大地保留了它们仍然存在的热稳定性。对于甲酚酶活性，藻酸盐珠的催化效率值（Vmax / Km）约为藻酸盐+κ-角叉菜胶珠的催化效率的两倍半。这些值对于儿茶酚酶活性非常接近。固定的珠子在4°C下保存30天后保存了它们的两种活性。

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