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Embryonic and Larval Development of Endangered Temoleh, Probarbus jullieni (Sauvage)

机译:濒临灭绝的Temoleh,Probarbus jullieni(Sauvage)的胚胎和幼虫发育

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The present study was carried out to investigate the embryonic and larval development of the endangered Temoleh, Probarbus jullieni in lab-rearing condition. The matured egg and sperm were collected by stripping the bloodstock of Temoleh fish after injecting with ovaprim hormone extract. The samples were collected from hatching tank at every 10 min interval for the first hour, 20 min for the second hour, 30 min for the third hour and then hourly interval up to hatching. After hatching, larvae were observed daily until the complete disappearance of the yolk sacs. The fertilized eggs were spherical, demersal, adhesive and brownish-yellow in colour with a mean diameter of 2316 μm. First cleavage occurred within 10 min post-fertilization at temperature ranged from 26.0 to 28.0°C. Hatching started 22 h post-fertilization and completed within 25 h at the same temperature. The yolk sac was completely absorbed 61 h after hatching. At the same time, the larvae started to swim actively and feed exogenously. This marks the first description on the early life history of P. jullieni . The present study will provide some valuable information on the ontogeny, breeding biology and early larval rearing protocol of P. jullieni which will ultimately be helpful towards the establishment of large scale seed production technique for conservation and aquaculture production.
机译:本研究旨在研究濒临灭绝的Temoleh,Probarbus jullieni在实验室饲养条件下的胚胎和幼虫发育。注入卵清蛋白提取物后,通过剥去Temoleh鱼的血液,收集成熟的卵和精子。在第一个小时内每隔10分钟从孵化槽中收集样品,第二小时每20分钟收集一次,第二小时每30分钟收集一次,然后每小时间隔直至孵化。孵化后,每天观察幼虫,直到卵黄囊完全消失。受精卵呈球形,深海形,黏性和棕黄色,平均直径为2316μm。第一次切割发生在受精后10分钟内,温度范围为26.0至28.0°C。受精后22小时开始孵化,并在相同温度下于25小时内完成孵化。卵卵囊在孵化后61 h被完全吸收。同时,幼虫开始活跃游动并外生觅食。这标志着对朱利安体育早期生活史的首次描述。本研究将提供一些有价值的信息,有关朱利安假单胞菌的个体发育,育种生物学和早期幼体饲养方案,这最终将有助于建立用于保护和水产养殖的大规模种子生产技术。

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