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首页> 外文期刊>Asian journal of animal and veterinary advances >Changes in Different Insulin Sensitive Tissues Gene Expression of Cat Fed on High-fat Diet
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Changes in Different Insulin Sensitive Tissues Gene Expression of Cat Fed on High-fat Diet

机译：高脂饮食喂猫的不同胰岛素敏感性组织基因表达的变化

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The objective of this study was to determine whether Peripheral Blood Leukocytes (PBL) are sensitive enough to detect early signs of diet induced obesity related changes occurring in insulin sensitive tissues, such as abdominal omental and subcutaneous adipose, liver and skeletal muscle, by comparing transcriptome profiles of insulin signaling (IRS-1, IRS-2 and PI3-K p85 α), adiponectin signaling (AdipoR1 and AdipoR2), energy homoeostasis (G6PDH and MDH) and sterol metabolism (FASN) genes as determined by RT-PCR in cats fed on High Fat (HF) diet. Regarding PBL concordance, using a HF diet induced obesity cat model, out of seven genes examined, concordance was observed with ~60% (5 out of 8) of them (IRS-1, IRS-2, Adipo-R1, Adipo-R2 and MDH) between PBL and tissue transcriptomes. HF diet cat PBL IRS-1 and IRS-2 mRNA expression were both reduced, when compared to control diet which was in concordance with reduced IRS-1 and IRS-2 mRNA expression in both abdominal and subcutaneous adipose of HF diet cats. Similar to IRS-1 and IRS-2, AdipoR1 and AdipoR2 mRNA expression in HF diet cats was also reduced, when compared to control diet which was in concordance with reduced AdipoR1 and AdipoR2 in liver and skeletal muscle, respectively of HF diet cats. Lastly, PBL MDH mRNA expression was reduced and was concordant with reduced mRNA expression in liver and skeletal muscle. Overall, our results demonstrate that PBL are sufficiently sensitive to high fat diet induced alterations to transcriptomes of insulin sensitive tissues and can serve as surrogate tissue for various insulin sensitive tissues.
机译：这项研究的目的是通过比较转录组来确定外周血白细胞（PBL）是否足够灵敏，以检测与饮食有关的肥胖相关变化的早期迹象，这些变化发生在胰岛素敏感组织（如腹膜和皮下脂肪，肝和骨骼肌）中RT-PCR测定猫的胰岛素信号转导（IRS-1，IRS-2和PI3-K p85α），脂联素信号转导（AdipoR1和AdipoR2），能量稳态（G6PDH和MDH）和固醇代谢（FASN）基因以高脂（HF）饮食为食。关于PBL一致性，使用HF饮食诱导的肥胖猫模型，在检查的七个基因中，观察到约60％的一致性（8个中的5个）（IRS-1，IRS-2，Adipo-R1，Adipo-R2）和MDH）在PBL和组织转录组之间。与对照饮食相比，HF饮食猫的PBL IRS-1和IRS-2 mRNA表达均降低，这与HF饮食猫的腹部和皮下脂肪中IRS-1和IRS-2 mRNA表达降低相一致。与IRS-1和IRS-2类似，与对照饮食相比，HF饮食猫的AdipoR1和AdipoR2 mRNA表达也降低了，这与HF饮食猫的肝脏和骨骼肌中AdipoR1和AdipoR2的减少相一致。最后，PBL MDH mRNA表达降低，并且与肝脏和骨骼肌中mRNA表达降低一致。总体而言，我们的结果表明，PBL对高脂饮食诱导的胰岛素敏感性组织转录组的改变具有足够的敏感性，并且可以作为各种胰岛素敏感性组织的替代组织。

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