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Effects of Coffee Ground Silage Feeding in Reducing Somatic Cell Count in Bovine Subclinical Mastitis Milk

机译:咖啡渣青贮饲料对减少牛亚临床乳腺炎牛奶中体细胞计数的影响

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bBackground and Objective:/b Bovine subclinical mastitis increases somatic cell count (SCC) without showing clinical symptoms, thus resulting in substantial economic losses to dairy farms. Numerous attempts to reduce SCC without using antibiotics have been reported but they have yet to be applied widely. This study investigated whether SCC can be reduced by feeding coffee ground silage containing large amounts of polyphenol, which is an inexpensive antioxidant to cows with subclinical mastitis. bMaterials and Methods:/b In cows with subclinical mastitis, the coffee ground silage feeding group was fed by adding 1.5 kg daySUP?1/SUP to normal feed and feed in the control group was not supplemented. Blood and milk were collected every week, the SCC in the quarter milk was measured and the oxygen radical absorbance capacity (ORAC) values in the blood and milk and the thiobarbit uric acid reactive substances (TBARS) value in milk were measured. In order to investigate the immune kinetics, leukocyte subpopulation analysis was performed for 7 days after feeding. bResults:/b Although the SCC did not show a significant decrease in the non-feeding group (n = 9), in the coffee ground silage feeding group (n = 11), it was decreased significantly (p0.01) at the 5th week. Furthermore, ORAC values were significantly higher (p0.05) in the coffee ground silage group (n = 5) than in the non-feeding group (n = 6), the TBARS value was significantly lower (p0.01). In addition, bulk milk SCC after feeding was also observed in 150 milking cattle in the summer season under heat stress . Furthermore, in the subpopulation of leukocytes after 7 days of feeding, there was a significant increase in CD4+cells and CD14+MHC class II+cells on day 1 after feeding. bConclusion:/b These results suggest that feeding of coffee ground silage to cows with subclinical mastitis increased the antioxidant activity and immune activity, leading to a decrease SCC in milk.
机译:背景和目的:牛亚临床乳腺炎会增加体细胞计数(SCC),而不会表现出临床症状,从而给奶牛场造成了巨大的经济损失。已经报道了许多不使用抗生素来减少SCC的尝试,但是它们尚未得到广泛应用。这项研究调查了是否可以通过饲喂含有大量多酚的咖啡粉青贮饲料来减少SCC,这对患有亚临床乳腺炎的母牛来说是一种廉价的抗氧化剂。 材料与方法:在患有亚临床乳腺炎的母牛中,咖啡渣青贮饲料组通过在正常饲料中添加1.5 kg day ?1 来喂养,而对照组则不添加补充。每周收集血液和牛奶,测量四分之一牛奶中的SCC,并测量血液和牛奶中的氧自由基吸收能力(ORAC)值以及牛奶中的硫代巴比妥尿酸反应性物质(TBARS)值。为了研究免疫动力学,进食后7天进行白细胞亚群分析。 结果:尽管非饲喂组(n = 9)的SCC没有显着下降,但咖啡渣青贮饲料组(n = 11)的SCC却显着降低(p < 0.01)在第5周。此外,咖啡渣青贮饲料组(n = 5)的ORAC值显着高于非喂养组(n = 6)(p <0.05),TBARS值显着较低(p <0.01)。此外,夏季在热应激下,在150头挤奶牛中还观察到了喂食后的散装牛奶SCC。此外,在进食7天后白细胞亚群中,进食后第1天CD4 +细胞和CD14 + MHC II类+细胞显着增加。 结论:这些结果表明,向患有亚临床性乳腺炎的母牛饲喂咖啡渣青贮饲料可提高其抗氧化活性和免疫活性,从而导致牛奶中的SCC降低。

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