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首页> 外文期刊>Arctic: Journal of the Arctic Institute of North America >An improved method for rapid propagation of Phalaenopsis hybrids via culture of longitudinally bisected shoot tips.
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An improved method for rapid propagation of Phalaenopsis hybrids via culture of longitudinally bisected shoot tips.

机译：一种改进的方法，可通过纵向对分的茎​​尖培养快速繁殖蝴蝶兰杂种。

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摘要

A new procedure for in vitro propagation of orchids belonging to the genus Phalaenopsis was developed. In contrast to the commonly employed micropropagation methodsthat make use of dormant flower stalk buds or intact shoot tips, we have used longitudinally bisected shoot tips derived from 3 – 3.5 month old axenic plantlets of Phalaenopsis hybrids for protocorm-like-bodies (PLB) induction. This procedure in combination with the use of New Dogashima Medium for PLB proliferation resulted in large-scale production of plantlets. Culturing the bisected shoot tips in half strength solid Murashige and Skoog medium supplemented with Thidiazuron (TDZ) (4.54μM–9.08 μM) and 10% coconut water resulted in early PLB induction in 20-25 days and callus-free formation of 13.6 – 15.2 shoot buds in 8 weeks. Intact shoot tips took 6-8 weeks to PLB induction and proliferated to a mean of 5.46 - 7 shoot buds per shoot tip in 12 - 16 weeks in different TDZ concentrations. Continuous multiplication of PLBs and shoot buds was maintained in New Dogashima Medium devoid of hormones by bimonthly subcultivation. About 50,000 hardened plantlets could be produced from a single flowerstalkwith 10 dormant buds, within 2 years from initiation of axenic shoot tip cultures.This protocol can be used to produce genetically stable Phalaenopsis orchids on a commercial scale.
机译：开发了一种新的兰花植物兰花的体外繁殖方法。与利用休眠的花梗芽或完整的芽梢的常用微繁殖方法相反，我们使用了从3-3.5个月大的蝴蝶兰杂种小植株衍生的纵向平分的芽梢来诱导原球茎样体（PLB）。此过程与使用新的多贺岛培养基进行PLB增殖相结合，导致了大规模生产小植株。在补充了噻唑啉酮（TDZ）（4.54μM–9.08μM）和10％椰子水的半强度固体Murashige和Skoog培养基中培养一分为二的芽尖，导致在20-25天内早期诱导PLB，无愈伤组织形成13.6 – 15.2。在8周内发芽。完整的芽尖需要6-8周的时间才能诱导PLB，并在不同的TDZ浓度下在12-16周内增殖至每个芽尖平均5.46-7个芽。通过每两个月一次的传代培养，在没有激素的新多a岛培养基中，PLB和芽的连续繁殖得以维持。从树桩芽培养开始的2年内，一个带有10个休眠芽的单一花茎可以生产大约50,000株硬化苗。该方案可用于商业规模生产遗传稳定的蝴蝶兰。

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