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Immobilization of paraoxonase onto chitosan and its characterization

机译：对氧磷酶固定在壳聚糖上及其表征

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Paraoxonase was covalently immobilized onto a glutaraldehyde containing amino group functionalized chitosan surface by chemical immobilization at pH 8.0. The amount of covalently bound hPON1 was found to be 32 mg/10 chitosan beads. The properties of immobilized enzyme were investigated and compared to those of free enzyme. The effects of various parameters such as pH, temperature, heat, and storage stability on immobilized enzyme were investigated. Kinetic parameters of the immobilized enzyme were also evaluated. Thermal and storage stability experiments were carried out. It was observed that the immobilized enzyme had longer storage stability and retained 50 % of its initial activity during 26 days.
机译：通过在pH 8.0下化学固定，将对氧磷酶共价固定在含戊二醛的氨基官能化壳聚糖表面上。发现共价结合的hPON1的量为32mg / 10壳聚糖珠。研究固定化酶的性质，并将其与游离酶的性质进行比较。研究了pH，温度，热量和储存稳定性等各种参数对固定化酶的影响。还评估了固定化酶的动力学参数。进行了热和储存稳定性实验。观察到固定化的酶具有更长的储存稳定性，并在26天内保留了其初始活性的50％。

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