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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Immobilization of laccase on polyacrylamide and polyacrylamide – κ – carragennan-based semi-interpenetrating polymer networks
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Immobilization of laccase on polyacrylamide and polyacrylamide – κ – carragennan-based semi-interpenetrating polymer networks

机译：漆酶在聚丙烯酰胺和聚丙烯酰胺-κ-卡拉格南系的半互穿聚合物网络上的固定化。

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摘要

In this study, laccase enzyme (L) from Agaricus bisporus was immobilized by entrapment into polyacrylamide (PAAm) and semi-interpenetrating polymer networks (semi-IPNs) prepared with either polyacrylamide/κ-carragennan (0.05g) [PAAm/ κ-car (0.05)] or polyacrylamide/κ-carragennan (0.1 g) [PAAm/ κ-car (0.1)]. The optimum pH was 6.0 for free L, 8.0 for PAAm-L, 8.5 for PAAm/κ-car (0.05)-L, and 9.0 for PAAm/κ-car (0.1)-L. The optimum temperature was determined as 45°C for free L and 60°C for all immobilized laccases. After 27 days of storage at 4°C, free enzyme lost its initial activity whereas immobilized enzymes retained 56 % ?80% of their initial activities. The immobilized samples were used repeatedly 35 times by retaining 28 %–58 % of their initial activity. Km(app) values were calculated as 0.088, 0.139, 0.133, and 0.131 mM and Vmax values were found to be 2.83 x 10?3, 4.51×10?3, 4.76×10?3, and 4.97×10?3 mM min?1 for free L and PAAm-L, PAAm/κ-car (0.05)-L, and PAAm/κ-car (0.1)-L, respectively.
机译：在这项研究中，将双孢蘑菇的漆酶（L）固定在聚丙烯酰胺（PAAm）和由聚丙烯酰胺/κ-卡拉胶聚糖（0.05g）制备的半互穿聚合物网络（semi-IPNs）中[PAAm /κ-car （0.05）]或聚丙烯酰胺/κ-卡拉胶（0.1 g）[PAAm /κ-car（0.1）]。游离L的最佳pH值为6.0，PAAm-L的最佳pH为8.PAAm /κ-car（0.05）-L为8.5，PAAm /κ-car（0.1）-L为9.0。游离L的最佳温度确定为45℃，所有固定的漆酶的最佳温度确定为60℃。在4°C下储存27天后，游离酶失去了其初始活性，而固定化酶则保留了其初始活性的56％〜80％。固定的样品可保留其初始活性的28％–58％，重复使用35次。 Km（app）值计算为0.088、0.139、0.133和0.131 mM，发现Vmax值为2.83 x 10？3、4.51×10？3、4.76×10？3和4.97×10？3 mM min游离L和PAAm-L，PAAm /κ-car（0.05）-L和PAAm /κ-car（0.1）-L分别为？1。

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