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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Glucose biosensor based on the immobilization of glucose oxidase on electrochemically synthesized polypyrrole-poly(vinyl sulphonate) composite film by cross-linking with glutaraldehyde
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Glucose biosensor based on the immobilization of glucose oxidase on electrochemically synthesized polypyrrole-poly(vinyl sulphonate) composite film by cross-linking with glutaraldehyde

机译：基于葡萄糖氧化酶固定化的戊二醛交联电化学合成聚吡咯-聚（乙烯基磺酸）复合膜的葡萄糖生物传感器

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In this study, a novel amperometric glucose biosensor was developed by immobilizing glucose oxidase (GOX) by cross-linking via glutaraldehyde on electrochemically polymerized polypyrrole-poly(vinyl sulphonate) (PPy–PVS) films on the surface of a platinum (Pt) electrode. Electropolymerization of pyrrole and poly(vinyl sulphonate) on the Pt surface was carried out with an electrochemical cell containing pyrrole and poly(vinyl sulphonate) by cyclic voltammetry between ?1.0 and +?2.0 V (vs.Ag/AgCl) at a scan rate of 50 mV/s upon the Pt electrode. The amperometric determination was based on the electrochemical detection of H2O2 generated in enzymatic reaction of glucose. Determination of glucose was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum parameters were found to be 7.5 and 65°C, respectively. The effect of working potential was investigated and optimum potential was determined to be 0.4 V. The operational stability of the enzyme electrode was also studied. The response of the PPy/PVS-GOX glucose biosensor exhibited good reproducibility with a relative standard deviation (RSD) of 2.48%. The glucose biosensor retained 63% of initial activity after 93 days when stored in 0.1 M phosphate buffer solution of pH 7.5 at 4°C. With the low operating potential, the biosensor demonstrated little interference from the possible interferants.
机译：在这项研究中，通过将戊二醛交联固定在铂（Pt）电极表面上的电化学聚合的聚吡咯-聚（乙烯基磺酸）（PPy-PVS）膜上，通过固定葡萄糖氧化酶（GOX），开发了一种新型的电流型葡萄糖生物传感器。吡咯和聚乙烯磺酸盐在Pt表面上的电聚合是通过循环伏安法在1.0和+2.0 V（vs.Ag/AgCl）之间的循环伏安法在扫描速率下用含有吡咯和聚乙烯磺酸盐的电化学电池进行的在Pt电极上的电压为50 mV / s。安培法测定是基于对葡萄糖的酶促反应中生成的H2O2的电化学检测。葡萄糖的测定是通过在0.4 V相对于Ag / AgCl的条件下氧化酶法生成的H2O2进行的。研究了pH和温度的影响，发现最佳参数分别为7.5和65°C。研究了工作电位的影响，确定最佳电位为0.4V。还研究了酶电极的操作稳定性。 PPy / PVS-GOX葡萄糖生物传感器的响应具有良好的可重复性，相对标准偏差（RSD）为2.48％。当将葡萄糖生物传感器保存在4°C的pH值为7.5的0.1 M磷酸盐缓冲溶液中后，在93天后仍保留了63％的初始活性。由于操作电位低，生物传感器显示出几乎不受可能的干扰物干扰。

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