Temperature Effects on Enzyme Activity of Chicken Liver Esterase Used in Calorimetric Biosensor

Yi-Hua Zheng; Jun Liu; Yong-Zhi Ma; Yi Xu; Fei Xu; Tse-Chao Hua

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Temperature Effects on Enzyme Activity of Chicken Liver Esterase Used in Calorimetric Biosensor

机译：温度对量热传感器中鸡肝酯酶活性的影响

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The temperature effects on enzyme activity at the conditions with and without inhibition, operation, and storage were investigated for free esterase and immobilized chicken liver esterase with three methods (adsorption, adsorption-microcapsule, and adsorption-crosslinking) used in a calorimetric biosensor. The results indicated that the temperature had significant effects on the enzyme activity by means of catalytic reaction, irreversible denaturation, mass transfer, and structure deformation of resin. Among all the esterase, the adsorption-crosslinking immobilized esterase had the best performances of enzyme activity retention ratio (EARR), above 96% in operation and 90% in storage on condition of maintaining its response to dichlorvos.

机译：用量热生物传感器中使用的三种方法（吸附，吸附微囊和吸附交联）研究了游离酯酶和固定化鸡肝酯酶在有无抑制，操作和存储条件下温度对酶活性的影响。结果表明，温度通过催化反应，不可逆变性，传质和树脂结构变形对酶的活性有显着影响。在所有酯酶中，吸附-交联固定化酯酶具有最佳的酶活性保留率（EARR），在维持其对敌敌畏的响应的条件下，其操作性高于96％，存储性高于90％。

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《Artificial cells, blood substitutes, and immobilization biotechnology.》 |2012年第2期|共7页
作者
Yi-Hua Zheng; Jun Liu; Yong-Zhi Ma; Yi Xu; Fei Xu; Tse-Chao Hua;
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中图分类生物医学工程;
关键词
Calorimetric biosensorTemperatureStabilityChicken liver esteraseImmobilizationAdsorptionAdsorption-microcapsuleAdsorption-crosslinking;

机译：量热生物传感器温度稳定性鸡肝酯酶固定化吸附吸附微胶囊吸附交联;

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1. Temperature effects on enzyme activity of chicken liver esterase used in calorimetric biosensor [J] . ZhengY.-H., LiuJ., MaY.-Z., Artificial cells, blood substitutes, and immobilization biotechnology . 2012,第1a2期

机译：温度对量热传感器中鸡肝酯酶酶活性的影响
2. Temperature Effects on Enzyme Activity of Chicken Liver Esterase Used in Calorimetric Biosensor [J] . Yi-Hua Zheng, Jun Liu Yong-Zhi Ma Artificial cells, blood substitutes, and immobilization biotechnology . 2012,第2期

机译：温度对量热传感器中鸡肝酯酶酶活性的影响
3. Temperature effects on enzyme activity of chicken liver esterase used in calorimetric biosensor. [J] . Yi-Hua Zheng, Jun Liu, Yong-Zhi Ma, Artificial cells, blood substitutes, and biotechnology . 2012,第1a2期

机译：温度对量热传感器中鸡肝酯酶酶活性的影响。
4. Effects of Driving Stress on Enzyme Activity and Elements Content in Blood Serum and Elements Content in Liver in Chicken [C] . . 2006

机译：驾车压力对鸡肉酶活性，血中元素含量和肝中元素含量的影响
5. Effects of Hormones, Dietary Carbohydrate Level and Temperature on the Expression of Key Enzymes in Carbohydrate Metabolism in the Liver of Silver Sea Bream (Sparus sarba) [D] . Leung, Ling Yan 2009

机译：激素，日粮碳水化合物水平和温度对银鲷鱼肝代谢糖代谢关键酶表达的影响
6. The effects of Mg2+ on certain steps in the mechanisms of the dehydrogenase and esterase reactions catalysed by sheep liver aldehyde dehydrogenase. Support for the view that dehydrogenase and esterase activities occur at the same site on the enzyme. [O] . F M Dickinson, G W Haywood 1986

机译：Mg2 +对绵羊肝醛脱氢酶催化的脱氢酶和酯酶反应机理中某些步骤的影响。支持脱氢酶和酯酶活性发生在酶的相同位点的观点。
7. The effects of Mg2+ on certain steps in the mechanisms of the dehydrogenase and esterase reactions catalysed by sheep liver aldehyde dehydrogenase. Support for the view that dehydrogenase and esterase activities occur at the same site on the enzyme. [O] . Dickinson, F M, Haywood, G W 1986

机译：Mg2 +对绵羊肝醛脱氢酶催化的脱氢酶和酯酶反应机理中某些步骤的影响。支持脱氢酶和酯酶活性发生在酶的相同位点的观点。

Temperature Effects on Enzyme Activity of Chicken Liver Esterase Used in Calorimetric Biosensor

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