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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Protective effect of dextrans on glucose oxidase denaturation and inactivation
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Protective effect of dextrans on glucose oxidase denaturation and inactivation

机译：葡聚糖对葡萄糖氧化酶变性和失活的保护作用

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摘要

In the present study, the stabilizing effect of dextrans as additives on the denaturation and inactivation of glucose oxidase (GOD) was investigated. Three different molecular weighted dextrans (Mw 17.5, 75, 188 kD) were used with different concentrations. Dramatically increased enzyme activities were measured after one hour of incubation of enzyme with additives between 25–40°C in water bath. Highest activity value was measured with 75 kDa molecular weighted dextran (in concentration 30% w/v) at pH 5. Dextran as an additive supplied a long shelf-life to the enzyme at 4°C. In the presence of the 75 kDa dextran, the enzyme was more stable and its activity was increased 2.7-fold at 30°C. In addition, dextran protected GOD against inactivation by a n-heptane/aqueous buffer-stirred system.
机译：在本研究中，研究了葡聚糖作为添加剂对葡萄糖氧化酶（GOD）变性和失活的稳定作用。使用了三种不同浓度的不同分子量的右旋糖酐（Mw 17.5、75、188 kD）。在水浴中，将酶与添加剂在25–40°C之间温育一小时后，酶活性显着提高。在pH 5下，使用75 kDa分子量的右旋糖酐（浓度为30％w / v）测量了最高活性值。右旋糖酐作为添加剂在4°C下为酶提供了较长的保存期限。在75 kDa葡聚糖的存在下，该酶更加稳定，在30°C下其活性增加了2.7倍。此外，右旋糖酐可保护GOD免受正庚烷/缓冲水溶液搅拌系统的灭活作用。

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