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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Preparation of a Polypyrrole-Polyvinylsulphonate Composite Film Biosensor for Determination of Phenol Based on Entrapment of Polyphenol Oxidase
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Preparation of a Polypyrrole-Polyvinylsulphonate Composite Film Biosensor for Determination of Phenol Based on Entrapment of Polyphenol Oxidase

机译：基于多酚氧化酶截留法测定酚类的聚吡咯-聚乙烯磺酸酯复合膜生物传感器的制备

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: In this paper, a novel amperometric phenol biosensor with immobilization of polyphenol oxidase (tyrosinase) on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique on the surface of a platinum electrode. The amperometric determination is based on the electrochemical reduction of quinon generated in the enzymatic reaction of phenol. The effects of pH and temperature were investigated and optimum parameters were found to be 8.0 and 30 °C, respectively. The linear working range of the electrode was 1.0 × 10?7 – 5.0 × 10?6 M. The storage stability and operation stability of the enzyme electrode were also studied.
机译：：在本文中，通过将捕获的多酚氧化酶（酪氨酸酶）固定在电化学聚合的聚吡咯-聚乙烯基磺酸盐（PPy-PVS）膜上，完成了一种新型的安培型苯酚生物传感器，该方法是通过在铂电极表面上进行包埋技术实现的。安培法的测定是基于苯酚的酶促反应中生成的醌的电化学还原。研究了pH和温度的影响，发现最佳参数分别为8.0和30°C。电极的线性工作范围为1.0×10？7 – 5.0×10？6M。还研究了酶电极的储存稳定性和操作稳定性。

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