首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >A Biosensor for the Determination of β-galactosidase Activity: A Different Viewpoint on Biosensors
【24h】

A Biosensor for the Determination of β-galactosidase Activity: A Different Viewpoint on Biosensors

机译:用于确定β-半乳糖苷酶活性的生物传感器:对生物传感器的不同观点

获取原文
           

摘要

: β-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor β-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. β-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for β-galactosidase detection was in the range of 0.0118 U mL?1to 0.47 U mL?1and a shorter response time of 50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of β-galactosidase activity.
机译::β-半乳糖苷酶将乳糖分解为葡萄糖和半乳糖。由于其对生物技术的兴趣,我们提出了一种生物传感器系统来监测β-半乳糖苷酶的活性。生物传感器的固定步骤通过循环伏安法和电化学阻抗谱进行鉴定。在柠檬酸盐缓冲溶液(0.05 M,pH 4.8)中以约+150 mV(相对于Ag / AgCl)的伏安法检测到β-半乳糖苷酶。 β-半乳糖苷酶检测的线性响应范围为0.0118 U mL?1至0.47 U mL?1,响应时间更短,为50 s。我们的结果表明,该生物传感器的电化学性质和分析特性对于监测β-半乳糖苷酶的活性非常有用和有效。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号