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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >A Novel Biosensor Based on Glucose Oxidase for Activity Determination of α – Amylase
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A Novel Biosensor Based on Glucose Oxidase for Activity Determination of α – Amylase

机译：基于葡萄糖氧化酶的新型生物传感器测定α-淀粉酶的活性

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: A glucose oxidase-based biosensor was developed for the determination of α-amylase activity. The determination method is based on monitoring the decrease in dissolved oxygen concentration related to the starch concentration, for which starch gives a reaction with α-amylase. Optimization parameters, including glucose oxidase amount, gelatin amount, and glutaraldehyde percentage for cross-linking, were investigated. The effects of pH, buffer system, and temperature on the biosensor system were also investigated. The biosensor had a linear relation to α-amylase activity and good measurement correlation between 0.66 and 9.83 U/ml. In sample analysis studies, α-amylase activity in baker's yeast was determined by the biosensor.
机译：：开发了一种基于葡萄糖氧化酶的生物传感器，用于测定α-淀粉酶的活性。该确定方法基于监测与淀粉浓度有关的溶解氧浓度的降低，为此淀粉与α-淀粉酶发生反应。研究了优化参数，包括葡萄糖氧化酶的量，明胶的量和用于交联的戊二醛的百分比。还研究了pH，缓冲系统和温度对生物传感器系统的影响。该生物传感器与α-淀粉酶活性具有线性关系，并且在0.66和9.83 U / ml之间具有良好的测量相关性。在样品分析研究中，通过生物传感器确定了面包酵母中的α-淀粉酶活性。

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