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首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >Conformal Nano-Thin Modified Polyelectrolyte Coatings for Encapsulation of Cells
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Conformal Nano-Thin Modified Polyelectrolyte Coatings for Encapsulation of Cells

机译：用于封装细胞的保形纳米薄改性聚电解质涂层

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: Encapsulation of cells in polymeric shells allows for separation of biological material from produced factors, which may find biotechnological and biomedical applications. Human T-lymphocyte cell line Jurkat as well as rat pancreatic islets were encapsulated using LbL technique within shells of polyelectrolyte modified by incorporation of biotin complexed with avidin to improve cell coating and to create the potential ability to elicit specific biochemical responses. The coating with nano-thin modified shells allowed for maintenance of the evaluated cells’ integrity and viability during the 8-day culture. The different PE impact may be observed on different biological materials. The islets exhibited lower mitochondrial activity than the Jurkat cells. Nevertheless, coating of cells with polyelectrolyte modified membrane allowed for functioning of both model cell types: 10 μm leukemia cells or 150 μm islets during the culture. Applied membranes maintained the molecular structure during the culture period. The conclusion is that applied modified membrane conformation may be recommended for coating shells for biomedical purposes.
机译：：将细胞封装在聚合物壳中可将生物材料与产生的因子分离，这可能会发现生物技术和生物医学应用。使用LbL技术将人T淋巴细胞细胞系Jurkat和大鼠胰岛封装在聚电解质的壳层中，该壳层通过掺入与抗生物素蛋白复合的生物素来改善细胞涂层并产生引起特定生化反应的潜在能力。纳米薄修饰壳涂层可以在8天的培养过程中维持评估细胞的完整性和活力。在不同的生物材料上可能会观察到不同的PE影响。胰岛的线粒体活性比Jurkat细胞低。然而，用聚电解质修饰的膜覆盖细胞可以使两种模型细胞发挥功能：在培养过程中有10μm白血病细胞或150μm胰岛。在培养期间，施加的膜保持分子结构。结论是，为生物医学目的，可建议使用修饰的膜构象来涂覆外壳。

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