• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Artificial cells, blood substitutes, and immobilization biotechnology. >In Vitro and In Vivo Biocompatibility Investigation of Diamond-like Carbon Coated Nickel-titanium Shape Memory Alloy
【24h】

In Vitro and In Vivo Biocompatibility Investigation of Diamond-like Carbon Coated Nickel-titanium Shape Memory Alloy

机译：类金刚石碳涂层镍钛形状记忆合金的体外和体内生物相容性研究

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

: Objective: To investigate the biocompatibility of diamond-like carbon (DLC) coated nickel-titanium shape memory alloy (NiTi SMA) in vitro and in vivo. Methods: The in vitro study was carried out by co-culturing the DLC coated and uncoated NiTi SMA with bone marrow mesenchymal stem cells (MSCs), respectively, and the in vivo study was carried out by fixing the rabbits’ femoral fracture model by DLC coated and uncoated NiTi SMA embracing fixator for 4 weeks, respectively. The concentration of the cells, alkaline phosphatase (AKP), and nickel ion in culture media were detected, respectively, at the first to fifth day after co-culturing. The inorganic substance, osteocalcin, alkaline phosphatase (ALP), and tumor necrosis factor (TNF) in callus surrounding fracture and the Ni+ in muscles surrounding fracture site, liver and brain were detected 4 weeks postoperatively. Results: The in vitro study showed that the proliferation of MSCs and the expression of AKP in the DLC-coated group were higher than the uncoated group (P 0.05), while the uncoated group released more Ni2+ into the culture media than that in the coated group (P 0.05). The in vivo study revealed that the inorganic substance and AKP, osteocalcin, and TNF expression were significantly higher in the DLC coated NiTi SMA embracing fixator than that in the uncoated group (P 0.05). Ni2+ in liver, brain, and muscles surrounding the fracture were significantly lower in the DLC coated groups than that in the uncoated group (P 0.05). Conclusion: Nickel-titanium shape memory alloy coated by diamond-like carbon appears to have better biocompatibility in vitro and in vivo compared to the uncoated one.
机译：：目的：研究类金刚石碳（DLC）涂层镍钛形状记忆合金（NiTi SMA）在体外和体内的生物相容性。方法：将DLC包被的和未包被的NiTi SMA分别与骨髓间充质干细胞（MSCs）共培养进行体外研究，并通过DLC固定兔股骨骨折模型进行体内研究。涂层和未涂层​​的NiTi SMA环抱固定器分别放置4周。在共培养后的第一至第五天分别检测培养基中的细胞浓度，碱性磷酸酶（AKP）和镍离子。术后4周检测到骨折周围骨us中的无机物质，骨钙素，碱性磷酸酶（ALP）和肿瘤坏死因子（TNF），以及骨折部位，肝和脑周围肌肉中的Ni +。结果：体外研究表明，DLC包被组的MSCs增殖和AKP表达均高于未包被组（P <0.05），而未包被组向培养基中释放的Ni2 +高于未包被组。包被组（P <0.05）。体内研究表明，DLC包被的NiTi SMA环抱固定剂中无机物质和AKP，骨钙素和TNF的表达显着高于未包被组（P <0.05）。 DLC涂层组的骨折周围肝脏，大脑和肌肉中的Ni2 +明显低于未涂层组（P <0.05）。结论：与未涂覆的镍钛形状记忆合金相比，涂覆有类金刚石碳的镍钛形状记忆合金似乎在体内外具有更好的生物相容性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号